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. 1996 May;34(5):1184–1188. doi: 10.1128/jcm.34.5.1184-1188.1996

Detecting bunyaviruses of the Bunyamwera and California serogroups by a PCR technique.

G Kuno 1, C J Mitchell 1, G J Chang 1, G C Smith 1
PMCID: PMC228979  PMID: 8727900

Abstract

Many bunyaviruses of the Bunyamwera and California serogroups are medically important human pathogens. The development of an effective technique to detect the viruses by using molecular biologic tools, such as PCR, improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. In this study, we evaluated eight pairs of primers for reactivity with 44 viruses of the genus Bunyavirus, using a reverse transcriptase PCR technique. With a pair of serogroup-specific primers we designed, all viruses of the serogroups tested could be detected. Further, virus-specific primer pairs were identified for California encephalitis virus, Jamestown Canyon virus, La Crosse virus, and snowshoe hare virus for use in North America. Using this technique, we could detect one La Crosse virus-infected mosquito in a pool of 100 mosquitoes with undetectable plaque titers.

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Selected References

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  1. Artsob H., Spence L. P., Th'ng C. Enzyme-linked immunosorbent assay typing of California serogroup viruses isolated in Canada. J Clin Microbiol. 1984 Aug;20(2):276–280. doi: 10.1128/jcm.20.2.276-280.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beaty B. J., Calisher C. H. Bunyaviridae--natural history. Curr Top Microbiol Immunol. 1991;169:27–78. [PubMed] [Google Scholar]
  3. Bowen M. D., Jackson A. O., Bruns T. D., Hacker D. L., Hardy J. L. Determination and comparative analysis of the small RNA genomic sequences of California encephalitis, Jamestown Canyon, Jerry Slough, Melao, Keystone and Trivittatus viruses (Bunyaviridae, genus Bunyavirus, California serogroup). J Gen Virol. 1995 Mar;76(Pt 3):559–572. doi: 10.1099/0022-1317-76-3-559. [DOI] [PubMed] [Google Scholar]
  4. Butenko A. M., Vladimirtseva E. A., Lvov S. D., Calisher C. H., Karabatsos N. California serogroup viruses from mosquitoes collected in the USSR. Am J Trop Med Hyg. 1991 Sep;45(3):366–370. doi: 10.4269/ajtmh.1991.45.366. [DOI] [PubMed] [Google Scholar]
  5. Calisher C. H. Medically important arboviruses of the United States and Canada. Clin Microbiol Rev. 1994 Jan;7(1):89–116. doi: 10.1128/cmr.7.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Campbell W. P., Huang C. Detection of California serogroup viruses using universal primers and reverse transcription-polymerase chain reaction. J Virol Methods. 1995 May;53(1):55–61. doi: 10.1016/0166-0934(94)00176-h. [DOI] [PubMed] [Google Scholar]
  7. Dunn E. F., Pritlove D. C., Elliott R. M. The S RNA genome segments of Batai, Cache Valley, Guaroa, Kairi, Lumbo, Main Drain and Northway bunyaviruses: sequence determination and analysis. J Gen Virol. 1994 Mar;75(Pt 3):597–608. doi: 10.1099/0022-1317-75-3-597. [DOI] [PubMed] [Google Scholar]
  8. Hildreth S. W., Beaty B. J., Meegan J. M., Frazier C. L., Shope R. E. Detection of La Crosse arbovirus antigen in mosquito pools: application of chromogenic and fluorogenic enzyme immunoassay systems. J Clin Microbiol. 1982 May;15(5):879–884. doi: 10.1128/jcm.15.5.879-884.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klimas R. A., Thompson W. H., Calisher C. H., Clark G. G., Grimstad P. R., Bishop D. H. Genotypic varieties of La Crosse virus isolated from different geographic regions of the continental United States and evidence for a naturally occurring intertypic recombinant La Crosse virus. Am J Epidemiol. 1981 Jul;114(1):112–131. doi: 10.1093/oxfordjournals.aje.a113158. [DOI] [PubMed] [Google Scholar]
  10. Mitchell C. J., Lvov S. D., Savage H. M., Calisher C. H., Smith G. C., Lvov D. K., Gubler D. J. Vector and host relationships of California serogroup viruses in western Siberia. Am J Trop Med Hyg. 1993 Jul;49(1):53–62. doi: 10.4269/ajtmh.1993.49.53. [DOI] [PubMed] [Google Scholar]
  11. Morvan J. M., Digoutte J. P., Marsan P., Roux J. F. Ilesha virus: a new aetiological agent of haemorrhagic fever in Madagascar. Trans R Soc Trop Med Hyg. 1994 Mar-Apr;88(2):205–205. doi: 10.1016/0035-9203(94)90297-6. [DOI] [PubMed] [Google Scholar]
  12. Pinheiro F. P., Travassos da Rosa A. P., Travassos da Rosa J. F., Ishak R., Freitas R. B., Gomes M. L., LeDuc J. W., Oliva O. F. Oropouche virus. I. A review of clinical, epidemiological, and ecological findings. Am J Trop Med Hyg. 1981 Jan;30(1):149–160. [PubMed] [Google Scholar]
  13. Roberts A., Rossier C., Kolakofsky D., Nathanson N., Gonzalez-Scarano F. Completion of the La Crosse virus genome sequence and genetic comparisons of the L proteins of the Bunyaviridae. Virology. 1995 Jan 10;206(1):742–745. doi: 10.1016/s0042-6822(95)80001-8. [DOI] [PubMed] [Google Scholar]
  14. Rosen L., Gubler D. The use of mosquitoes to detect and propagate dengue viruses. Am J Trop Med Hyg. 1974 Nov;23(6):1153–1160. doi: 10.4269/ajtmh.1974.23.1153. [DOI] [PubMed] [Google Scholar]
  15. Urquidi V., Bishop D. H. Non-random reassortment between the tripartite RNA genomes of La Crosse and snowshoe hare viruses. J Gen Virol. 1992 Sep;73(Pt 9):2255–2265. doi: 10.1099/0022-1317-73-9-2255. [DOI] [PubMed] [Google Scholar]
  16. Vodkin M. H., Streit T., Mitchell C. J., McLaughlin G. L., Novak R. J. PCR-based detection of arboviral RNA from mosquitoes homogenized in detergent. Biotechniques. 1994 Jul;17(1):114–116. [PubMed] [Google Scholar]
  17. Wasieloski L. P., Jr, Rayms-Keller A., Curtis L. A., Blair C. D., Beaty B. J. Reverse transcription-PCR detection of LaCrosse virus in mosquitoes and comparison with enzyme immunoassay and virus isolation. J Clin Microbiol. 1994 Sep;32(9):2076–2080. doi: 10.1128/jcm.32.9.2076-2080.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yuill T. M. Reduction of arbovirus plaque numbers by suspensions of normal mosquitoes. Am J Trop Med Hyg. 1969 Jul;18(4):609–613. doi: 10.4269/ajtmh.1969.18.609. [DOI] [PubMed] [Google Scholar]

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