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. 1996 Sep 1;37(3):251–263. doi: 10.1186/BF03548092

Intrauterine Bacterial Findings and Hormonal Profiles in Post-partum Cows with Normal Puerperium

Förekomst av intrauterina bakterier och hormonprofiler hos postpartala mjölkkor med ett normalt puerperium

M Bekana 14, P Jonsson 24, H Kindahl 14,✉
PMCID: PMC8063984  PMID: 8996871

Abstract

The post-partum intrauterine bacterial flora, prostaglandin release, uterine involution and resumption of ovarian activity were studied in 9 Swedish dairy cows during the first 8-week period. Uterine involution was monitored by transrectal examinations of the reproductive tract 3 times weekly. Bacteriological examination was performed from twice weekly uterine biopsies. The main PGF2α metabolite (15-ketodi- hydro-PGF2α) was monitored from twice daily blood plasma samples, while morning samples were used for progesterone determinations. The cows were assigned to 2 groups: Group I (n = 7) with an uncomplicated puerperal period and Group II (n = 2) with signs of intrauterine infections. A total of 143 biopsies were collected, of which 129 (90.2%) were found to be bacteriologically negative. Thirteen (9.1%) of the remaining 14 biopsies were bacteriologically positive, while one (0.7%) was probably a contamination on a single occasion. The 13 bacteriologically positive biopsies belonged to the Group II cows from which 31 isolates contained 6 different genera of facultative and obligate anaerobic bacteria. Actinomyces pyogenes along with Bacteroides sp. and Fusobacterium necrophorum were found to predominate in a mixed flora. The bacteria were rapidly eliminated and disappeared completely from the uteri towards the end of the third week post-partum. The average number of days required for completion of uterine involution was 21.8 ± 3.0 for all animals. The plasma levels of the PGF2α metabolite were significantly elevated for the first 12–18, and 18 and 27 days in Group I and Group II, respectively. There was no significant relationship between the duration of PGF2α release and the time required for completion of uterine involution (p>0.05). Progesterone analysis showed resumption of ovarian activity and subsequent ovulation in 4 of the 9 cows 44-55 days post-partum. Thus, intrauterine infections are not commonly seen in cows with normal calving and comparison between the duration of PGF2α release and the time required for completion of uterine involution showed insignificant correlation. However, the longer duration of PGF2α release recorded in the 2 cows with intrauterine infections are related to the increased frequency of infections.

Keywords: prostaglandin, progesterone, uterine biopsy, uterine involution

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Acknowledgment

This study was financially supported by grants from the Swedish Council for Forestry and Agricultural Research. The authors wish to convey their appreciation to Miss Boel Brändström for her skilful assistance in identifying the bacteria.

References

  1. Bekana M, Ekman T, Kindahl H. Ultrasonography of the bovine post-partum uterus with retained foetal membranes. J. Vet. Med. A. 1994;41:653–662. doi: 10.1111/j.1439-0442.1994.tb00133.x. [DOI] [PubMed] [Google Scholar]
  2. Bekana M, Jonsson P, Ekman T, Kindahl H. Intrauterine bacterial findings in post-partum cows with retained foetal membranes. J. Vet. Med. A. 1994;41:663–670. doi: 10.1111/j.1439-0442.1994.tb00134.x. [DOI] [PubMed] [Google Scholar]
  3. Bekana M, Odensvik K, Kindahl H. Prostaglandin F2a metabolite and progesterone profiles in postpartum cows with retained foetal membranes. Acta vet. scand. 1996;37:171–185. doi: 10.1186/BF03548110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berglund B, Philipsson J. The influence of relative birth weight and certain other factors on calving performance in Swedish dairy cattle breeds. Anim. Reprod. Sci. 1987;15:81–93. doi: 10.1016/0378-4320(87)90007-8. [DOI] [Google Scholar]
  5. Bolinder A, Seguin B, Kindahl H, Bouley D, Otterby D. Retained foetal membranes in cows: manual removal versus non-removal and its effect on reproductive performance. Theriogenology. 1988;30:45–55. doi: 10.1016/0093-691X(88)90262-2. [DOI] [PubMed] [Google Scholar]
  6. Bosu WTK, Liptrap RM, Kenneth EL. Peripheral changes in plasma progesterone and 15-keto-13,14-dihydro-prostaglandin F2a concentrations in Holstein cows with or without retained foetal membranes. Anim. Reprod. Sci. 1984;7:497–510. doi: 10.1016/0378-4320(84)90054-X. [DOI] [Google Scholar]
  7. Bretzlaff KN, Whitmore HL, Spahr SL, Ott RS. Incidence and treatments of postpartum reproductive problems in a dairy herd. Theriogenology. 1982;17:527–535. doi: 10.1016/0093-691X(82)90178-9. [DOI] [PubMed] [Google Scholar]
  8. De Bois CHW. Endometritis en vruchtbaarheid bij het rund. 1961. p. 145. [Google Scholar]
  9. Edqvist L-E, Kindahl H, Stabenfeldt GH. Release of prostaglandin F2a during the bovine peripartal period. Prostaglandins. 1978;16:111–119. doi: 10.1016/0090-6980(78)90207-1. [DOI] [PubMed] [Google Scholar]
  10. Elliott L, McMahon KJ, Gier HT, Marion GB. Uterus of the cow after parturition: bacterial content. Amer. J. vet Res. 1968;29:77–81. [PubMed] [Google Scholar]
  11. Eriksson S, Sanne S, Thomke S. Fodermedlen (Feedstuff) 1972. p. 251. [Google Scholar]
  12. Forsberg M, Tagle R, Madej A, Molina JR, Carlsson MA. Radioimmunoassay of bovine, ovine and porcine luteinizing hormone with a monoclonal antibody and a human tracer. Acta. vet. scand. 1993;34:255–262. doi: 10.1186/BF03548189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fredriksson G, Kindahl H, Sandstedt K, Edqvist L-E. Intrauterine bacterial findings and release of PGF2a in the post-partum dairy cow. Zbl. Vet. Med. A. 1985;32:368–380. doi: 10.1111/j.1439-0442.1985.tb01953.x. [DOI] [PubMed] [Google Scholar]
  14. Fredriksson G, Kindahl H, Alenius S, Carlsson U, Cort N, Edqvist L-E, Uggla A. Hormonal and physiological factors which influence the establishment of infection in the uterus. 1988. [Google Scholar]
  15. Granström E, Kindahl H. Radioimmunoassay of the major plasma metabolite of PGF2a, 15-keto-13,14-dihydro-PGF2a. Methods Enzymol. 1982;86:320–339. doi: 10.1016/0076-6879(82)86204-6. [DOI] [PubMed] [Google Scholar]
  16. Griffin JFT, Hartigan PJ, Nunn WR. Nonspecific uterine infection and bovine fertility. 1. Infection patterns and endometritis during the first seven weeks post-partum. Theriogenology. 1974;1:91–106. doi: 10.1016/0093-691X(74)90052-1. [DOI] [PubMed] [Google Scholar]
  17. Griffin JFT, Murphy JA, Nunn WR, Hartigan PJ. Repetitive in vivo sampling of the bovine uterus under field conditions. Br. vet. J. 1974;130:259–264. doi: 10.1016/S0007-1935(17)35891-8. [DOI] [PubMed] [Google Scholar]
  18. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey's Manual of Determinative Bacteriology. 9th. Baltimore: Williams and Wilkins co; 1994. p. 299. [Google Scholar]
  19. Hunter MG. Characteristics and causes of the inadequate corpus luteum. J. Reprod. Fert. 1991;43(Suppl.):91–99. [PubMed] [Google Scholar]
  20. Hussain AM, Daniel RCW, Boyle O. Post-partum uterine flora following normal and abnormal Puerperium in cows. Theriogenology. 1990;34:291–302. doi: 10.1016/0093-691X(90)90522-U. [DOI] [PubMed] [Google Scholar]
  21. Kindahl H, Edqvist L-E, Larsson K, Malmqvist Influence of prostaglandins on ovarian function post-partum. Current Topics in Veterinary Medicine and Animal Science. 1982;20:173–196. [Google Scholar]
  22. Kindahl H, Fredriksson G, Madej A, Edqvist L-E. Proc. 10th Int. Congr. on Animal Reprod. and Art. In-sem. 1984. Role of prostaglandins in uterine involution; pp. 119–16. [Google Scholar]
  23. Kindahl H, Cort N, Fredriksson G, Edqvist L-E, Stabenfeldt G. Proc. Int. Symp. on the Use of Nuclear Techniques in Studies of Animal Production and Health in Different Environments. Vienna: Atomic Energy Agency, and the Food and Agric. Org. of the United Nations; 1986. Some aspects of the possible roles of diseases in altering reproductive performance; pp. 263–273. [Google Scholar]
  24. Kindahl H, Odensvik K, Aiumlamai S, Fredriksson G. Utero-ovarian relationships during the bovine post-partum period. Anim. Reprod. Sci. 1992;28:363–369. doi: 10.1016/0378-4320(92)90122-T. [DOI] [Google Scholar]
  25. Lamming GE, Wathes DC, Peters AR. J. Reprod. Fert. 1981. Endocrine patterns of the post-partum cow; pp. 155–170. [PubMed] [Google Scholar]
  26. Larsson K, Jansson L, Berglund B, Edqvist L-E, Kindahl H. Post-partum reproductive performance in dairy cows. Acta vet. scand. 1984;25:445–461. doi: 10.1186/BF03547259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Lindell J-O, Kindahl H, Jansson L, Edqvist L-E. Post-partum release of prostaglandin F2a and uterine involution in the cow. Theriogenology. 1982;17:237–245. doi: 10.1016/0093-691X(82)90085-1. [DOI] [PubMed] [Google Scholar]
  28. Madej A, Kindahl H, Woyno W, Edqvist L-E, Stup-nicki R. Blood levels of 15-keto-13,14-dihydro-prostaglandin F2a during the post-partum period in primiparous cows. Theriogenology. 1984;21:279–287. doi: 10.1016/0093-691X(84)90413-8. [DOI] [PubMed] [Google Scholar]
  29. Madej A, Kindahl H, Larsson K, Edqvist L-E. Sequential hormonal changes in the post-partum dairy cow. Acta vet. scand. 1986;27:280–287. doi: 10.1186/BF03548172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Markusfeld O. Acta vet. scand. 1993. Parturition disease complex of the high-yielding dairy cow; pp. 9–15. [PubMed] [Google Scholar]
  31. Messier S, Higgins R, Couture Y, Morin M. Comparison of swabbing and biopsy for studying the flora of the bovine post-partum uterus. Can. Vet. J. 1984;25:283–288. [PMC free article] [PubMed] [Google Scholar]
  32. Morrow DA, Roberts SJ, McEntee K. Post-partum ovarian activity and involution of the uterus and cervix in dairy cattle. 1. Ovarian activity. Cor. Vet. 1968;55:174–198. [PubMed] [Google Scholar]
  33. Olson JD, Ball L, Mortimer RG, Farin PW, Adeny WS, Huffman M. Aspects of bacteriology and endocri-nology of cows with pyometra and retained foetal membranes. Am. J. vet. Res. 1984;45:2251–2255. [PubMed] [Google Scholar]
  34. Peter AT, Bosu WTK. Effects of intrauterine infection on the function of the corpora lutea formed after first post-partum ovulations in dairy cows. Theriogenology. 1987;27:593–609. doi: 10.1016/0093-691X(87)90054-9. [DOI] [PubMed] [Google Scholar]
  35. Ruder CA, Sasser RG, Williams RJ, Ely JK, Bull RC, Butler JE. Uterine infections in the post-partum cow. II. Possible synergistic effect of Fusobacte-rium and Corynebacterium pyogenes. Theriogenology. 1981;75:573–579. [Google Scholar]
  36. Slama H, Vaillancourt D, Goff AK. Effect of bacterial cell wall and lipopolysacharide on arachidonic acid metabolism by caruncular and allantochor-ionic tissues from cows that calved normally and those that retained foetal membranes. Theriogenology. 1994;41:923–942. doi: 10.1016/0093-691X(94)90508-G. [DOI] [PubMed] [Google Scholar]
  37. Spore W, Moskal PA, Nakamura RM, Mishell DR. Bacteriology of post-partum oviducts and endometrium. Amer. J. Obstet. Gynec. 1970;4:572–577. doi: 10.1016/S0002-9378(16)33944-8. [DOI] [PubMed] [Google Scholar]
  38. Thatcher WW, Guilbault LA, Collier RJ, Lewis GS, Drost M, Knickerbocker J, Foster DB, Wilcox CJ. The impact of antepartum physiology on postpartum performance in cows. Current Topics in Veterinary Medicine and Animal Science. 1982;20:3–24. [Google Scholar]
  39. Thompson FN, Page RD, Cook CB, Caudle AB. Prostaglandin F2a metabolite levels in normal and uterine-infected post-partum cows. Vet. Res. Comm. 1987;77:503–507. doi: 10.1007/BF00396367. [DOI] [PubMed] [Google Scholar]
  40. Zarco L, Stabenfeldt GH, Kindahl H, Quirke JF, Granström E. Persistence of luteal activity in the non-pregnant ewe. Anim. Reprod. Sci. 1984;7:245–267. doi: 10.1016/0378-4320(84)90038-1. [DOI] [Google Scholar]

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