Abstract
During feeding experiments with [omega-14C]oleic acid and [omega-14c]nervonic acid to adult rats, 14C-labelled C26, C28 and C30 fatty acids were recovered from the intestinal mucosa, liver, plasma, kidney and stools. The structures of these fatty acids were determined by g.l.c., radio-g.l.c. and mass spectrometry. The Schmidt and Ginger degradation methods indicated that most of the 14C found in these extra-long fatty acids remained in the omega position. These radioactive extra-long fatty acids were found mainly in the polar lipids of rats killed 3 or 15 h after being fed on labelled oleic acid or nervonic acid. Rats killed 63 h later yielded only traces of these extra-long fatty acids. When the rats were given antibiotics or received the same radioactive fatty acids by intravenous injection, the labelled extra-long fatty acids could not be detected in any of the tissues. We conclude that they were probably synthesized by elongation of oleic acid and nervonic acid by intestinal micro-organisms (probably yeasts) and then absorbed by the intestinal mucosa.
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- Chevallier F., Lutton C. Vitesses des processus de renouvellement du cholestérol contenu dans son espace de transfert, chez le rat. I. Méthodes et résultats obtenus dans le cas d'un régime semi-synthétique témoin. Bull Soc Chim Biol (Paris) 1966;48(4):507–524. [PubMed] [Google Scholar]
- FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
- MORRISON W. R., SMITH L. M. PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL. J Lipid Res. 1964 Oct;5:600–608. [PubMed] [Google Scholar]
- Marsh J. B., Weinstein D. B. Simple charring method for determination of lipids. J Lipid Res. 1966 Jul;7(4):574–576. [PubMed] [Google Scholar]
- Rabinowitz J. L., Bailey T. A., Marsh J. B. Polar and neutral lipids of pig gingiva. Arch Oral Biol. 1971 Oct;16(10):1195–1205. doi: 10.1016/0003-9969(71)90049-5. [DOI] [PubMed] [Google Scholar]
- Rabinowitz J. L. Determination of elongation of fatty acids. Anal Biochem. 1968 Mar;22(3):498–510. doi: 10.1016/0003-2697(68)90291-1. [DOI] [PubMed] [Google Scholar]
- Rabinowitz J. L., Hercker E. S. Incomplete oxidation of palmitate and leakage of intermediary products during anoxia. Arch Biochem Biophys. 1974 Apr 2;161(2):621–627. doi: 10.1016/0003-9861(74)90345-2. [DOI] [PubMed] [Google Scholar]
- Welch J. W., Burlingame A. L. Very long-chain fatty acids in yeast. J Bacteriol. 1973 Jul;115(1):464–466. doi: 10.1128/jb.115.1.464-466.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whereat A. F., Hull F. E., Orishimo M. W. The role of succinate in the regulation of fatty acid synthesis by heart mitochondria. J Biol Chem. 1967 Sep 25;242(18):4013–4022. [PubMed] [Google Scholar]