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Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1980 Jun;39(3):207–216. doi: 10.1136/ard.39.3.207

Synovial fluid beta 2 microglobulin and hydroxyproline fractions in rheumatoid arthritis and nonautoimmune arthropathies.

D Manicourt, H Brauman, S Orloff
PMCID: PMC1000518  PMID: 6158297

Abstract

Fourteen patients with classical and definite seropositive rheumatoid arthritis (RA), 5 patients with microcrystalline arthritis, and 7 patients with osteoarthrosis were studied with respect to markers of newly synthesised collagen (synovial NDOH pro levels); markers of connective tissue resorption (synovial DOH pro and NBH levels); markers of lymphoid tissue activity (synovial and plasma beta 2m levels). Higher amounts of NDOH pro in RA synovial fluid are compatible with the hypothesis of a local connective tissue production as suggested by Uitto et al. on basis of a higher protocollagen proline hydroxylase activity in RA synovial tissue. DOH pro and NBH do not differ significantly in synovial fluid from RA or gouty patients, but the correlations between these forms of OH pro and, respectively, synovial lymphocytes and polymorphonuclear leucocytes are indicative of different processes of connective tissue remodelling in the 2 conditions. Synovial beta 2m levels are a direct function of total synovial lymphocyte counts independently of the type of arthropathy being explored. The ratio of synovial to plasma beta 2m is systematically above unity in RA patients only.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berggård I., Bearn A. G. Isolation and properties of a low molecular weight beta-2-globulin occurring in human biological fluids. J Biol Chem. 1968 Aug 10;243(15):4095–4103. [PubMed] [Google Scholar]
  2. Bernier G. M., Fanger M. W. Synthesis of 2 -microglobulin by stimulated lymphocytes. J Immunol. 1972 Aug;109(2):407–409. [PubMed] [Google Scholar]
  3. Bishop M. C., Smith R. Non-protein-bound hydroxyproline in plasma in renal bone disease. Clin Chim Acta. 1971 Jul;33(2):403–408. doi: 10.1016/0009-8981(71)90499-2. [DOI] [PubMed] [Google Scholar]
  4. Bunch T. W., Hunder G. G., McDuffie F. C., O'Brien P. C., Markowitz H. Synovial fluid complement determination as a diagnostic aid in inflammatory joint disease. Mayo Clin Proc. 1974 Oct;49(10):715–720. [PubMed] [Google Scholar]
  5. Cejka J., Cohen F., Kithier K. Serum beta2-microglobulin levels in normal children and sex-linked agammaglobulinemia patients. Clin Chim Acta. 1973 Aug 17;47(1):59–61. doi: 10.1016/0009-8981(73)90059-4. [DOI] [PubMed] [Google Scholar]
  6. Cresswell P., Springer T., Strominger J. L., Turner M. J., Grey H. M., Kubo R. T. Immunological identity of the small subunit of HL-A antigens and beta2-microglobulin and its turnover on the cell membrane. Proc Natl Acad Sci U S A. 1974 May;71(5):2123–2127. doi: 10.1073/pnas.71.5.2123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cresswell P., Turner M. J., Strominger J. L. Papain-solubilized HL-A antigens from cultured human lymphocytes contain two peptide fragments. Proc Natl Acad Sci U S A. 1973 May;70(5):1603–1607. doi: 10.1073/pnas.70.5.1603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cunningham B. A., Wang J. L., Berggård I., Peterson P. A. The complete amino acid sequence of beta 2-microglobulin. Biochemistry. 1973 Nov 20;12(24):4811–4822. doi: 10.1021/bi00748a001. [DOI] [PubMed] [Google Scholar]
  9. Dayer J. M., Graham R., Russell G., Krane S. M. Collagenase production by rheumatoid synovial cells: stimulation by a human lymphocyte factor. Science. 1977 Jan 14;195(4274):181–183. doi: 10.1126/science.188134. [DOI] [PubMed] [Google Scholar]
  10. Delfosse L., Orloff S., Rao V. H., Strosberg E., Verbruggen L. Automated analysis of hydroxyproline in urine. Indian J Biochem Biophys. 1975 Sep;12(3):280–282. [PubMed] [Google Scholar]
  11. Evanson J. M., Jeffrey J. J., Krane S. M. Studies on collagenase from rheumatoid synovium in tissue culture. J Clin Invest. 1968 Dec;47(12):2639–2651. doi: 10.1172/JCI105947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Evrin P. E., Peterson P. A., Wide L., Berggård I. Radioimmunoassay of 2 -microglobulin in human biological fluids. Scand J Clin Lab Invest. 1971 Dec;28(4):439–443. doi: 10.3109/00365517109095721. [DOI] [PubMed] [Google Scholar]
  13. Evrin P. E., Wibell L. Serum 2 -microglobulin in various disorders. Clin Chim Acta. 1973 Jan 24;43(2):183–186. doi: 10.1016/0009-8981(73)90449-x. [DOI] [PubMed] [Google Scholar]
  14. Evrin P. E., Wibell L. The serum levels and urinary excretion of 2 -microglobulin in apparently healthy subjects. Scand J Clin Lab Invest. 1972 Feb;29(1):69–74. doi: 10.3109/00365517209081057. [DOI] [PubMed] [Google Scholar]
  15. Goulding A., McChesney R., Irvine R. O., Dixon S. R. Serum hydroxyproline and renal osteodystrophy in patients maintained on haemodialysis. N Z Med J. 1974 Dec 25;80(530):540–544. [PubMed] [Google Scholar]
  16. Grey H. M., Kubo R. T., Colon S. M., Poulik M. D., Cresswell P., Springer T., Turner M., Strominger J. L. The small subunit of HL-A antigens is beta 2-microglobulin. J Exp Med. 1973 Dec 1;138(6):1608–1612. doi: 10.1084/jem.138.6.1608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. HEDBERG H. STUDIES ON THE DEPRESSED HEMOLYTIC COMPLEMENT ACTIVITY OF SYNOVIAL FLUID IN ADULT RHEUMATOID ARTHRITIS. Acta Rheumatol Scand. 1963;9:165–193. [PubMed] [Google Scholar]
  18. Haddad J. G., Jr, Couranz S., Avioli L. V. Nondialyzable urinary hydroxyproline as an index of bone collagen formation. J Clin Endocrinol Metab. 1970 Mar;30(3):282–287. doi: 10.1210/jcem-30-3-282. [DOI] [PubMed] [Google Scholar]
  19. Harris E. D., Jr, DiBona D. R., Krane S. M. Collagenases in human synovial fluid. J Clin Invest. 1969 Nov;48(11):2104–2113. doi: 10.1172/JCI106177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hütteroth T. H., Cleve H., Litwin S. D., Poulik M. D. The relationship between 2 -microglobulin and immunoglobulin in cultured human lymphoid cell lines. J Exp Med. 1973 Mar 1;137(3):838–843. doi: 10.1084/jem.137.3.838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kaye M. Recognition of osteodystrophy in chronic uremia. Am J Med Sci. 1971 Apr;261(4):182–187. doi: 10.1097/00000441-197104000-00001. [DOI] [PubMed] [Google Scholar]
  22. Kithier K., Cejka J., Belamaric J., Al-Sarraf M., Peterson W. D., Jr, Vaitkevicius V. K., Poulik M. D. Beta2-microglobulin: occurrence in fetal life and malignancy. Clin Chim Acta. 1974 May 17;52(3):293–299. doi: 10.1016/0009-8981(74)90113-2. [DOI] [PubMed] [Google Scholar]
  23. Kivirikko K. I. Urinary excretion of hydroxyproline in health and disease. Int Rev Connect Tissue Res. 1970;5:93–163. doi: 10.1016/b978-0-12-363705-5.50008-7. [DOI] [PubMed] [Google Scholar]
  24. Krane S. M., Muñoz A. J., Harris E. D., Jr Collagen-like fragments: excretion in urine of patients with Paget's disease of bone. Science. 1967 Aug 11;157(3789):713–716. doi: 10.1126/science.157.3789.713. [DOI] [PubMed] [Google Scholar]
  25. Krane S. M., Muñoz A. J., Harris E. D., Jr Urinary polypeptides related to collagen synthesis. J Clin Invest. 1970 Apr;49(4):716–729. doi: 10.1172/JCI106284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Manicourt D., Brauman H., Orloff S. Plasma and urinary levels of beta2 microglobulin in rheumatoid arthritis. Ann Rheum Dis. 1978 Aug;37(4):328–332. doi: 10.1136/ard.37.4.328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Marx J. I. Immunology: Role of beta2-Microglobulin. Science. 1974 Aug 2;185(4149):428–429. doi: 10.1126/science.185.4149.428. [DOI] [PubMed] [Google Scholar]
  28. Nakamuro K., Tanigaki N., Pressman D. Multiple common properties of human beta2-microglobulin and the common portion fragment derived from HL-A antigen molecules. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2863–2865. doi: 10.1073/pnas.70.10.2863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Nilsson K., Evrin P. E., Berggård I., Pontén J. Involvement of lymphoid and nonlymphoid cells in the production of beta2-microglobulin-a homologue of the constant domains of IgG. Nat New Biol. 1973 Jul 11;244(132):44–45. doi: 10.1038/newbio244044a0. [DOI] [PubMed] [Google Scholar]
  30. Nilsson K., Evrin P. E., Welsh K. I. Production of beta 2-microglobulin by normal and malignant human cell lines and peripheral lymphocytes. Transplant Rev. 1974;21(0):53–84. doi: 10.1111/j.1600-065x.1974.tb01546.x. [DOI] [PubMed] [Google Scholar]
  31. Peterson P. A., Cunningham B. A., Berggård I., Edelman G. M. 2 -Microglobulin--a free immunoglobulin domain. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1697–1701. doi: 10.1073/pnas.69.7.1697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Peterson P. A., Rask L., Lindblom J. B. Highly purified papain-solubilized HL-A antigens contain beta2-microglobulin. Proc Natl Acad Sci U S A. 1974 Jan;71(1):35–39. doi: 10.1073/pnas.71.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Poulik M. D., Bloom A. D. Beta 2 -microglobulin production and secretion by lymphocytes in culture. J Immunol. 1973 May;110(5):1430–1433. [PubMed] [Google Scholar]
  34. Reid K. B., Lowe D. M., Porter R. R. Isolation and characterization of C1q, a subcomponent of the first component of complement, from human and rabbit sera. Biochem J. 1972 Dec;130(3):749–763. doi: 10.1042/bj1300749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Sagar S., Borra S., Kaye M. Thermolability of alkaline phosphatase in the evaluation of renal osteodystrophy. Nephron. 1971;8(3):270–277. doi: 10.1159/000179928. [DOI] [PubMed] [Google Scholar]
  36. Smithies O., Poulik M. D. Initiation of protein synthesis at an unusual position in an immunoglobulin gene? Science. 1972 Jan 14;175(4018):187–189. doi: 10.1126/science.175.4018.187. [DOI] [PubMed] [Google Scholar]
  37. Talal N., Grey H. M., Zvaifler N., Michalski J. P., Daniels T. E. Elevated salivary and synovial fluid beta2-microglobulin in Sjogren's syndrome and rheumatoid arthritis. Science. 1975 Mar 28;187(4182):1196–1198. doi: 10.1126/science.46621. [DOI] [PubMed] [Google Scholar]
  38. Tanigaki N., Pressman D. The basic structure and the antigenic characteristics of HL-A antigens. Transplant Rev. 1974;21(0):15–34. doi: 10.1111/j.1600-065x.1974.tb01544.x. [DOI] [PubMed] [Google Scholar]
  39. Uitto J., Lindy S., Turto H., Vainio K. Collagen biosynthesis in rheumatoid synovial tissue. J Lab Clin Med. 1972 Jun;79(6):960–971. [PubMed] [Google Scholar]
  40. Varghese Z., Moorhead J. F., Wills M. R. Plasma calcium and magnesium fractions in chronic-renal-failure patients on maintenance haemodialysis. Lancet. 1973 Nov 3;302(7836):985–988. doi: 10.1016/s0140-6736(73)91087-8. [DOI] [PubMed] [Google Scholar]
  41. Versey J. M., Hobbs J. R., Holt P. J. Complement metabolism in rheumatoid arthritis. I. Longitudinal studies. Ann Rheum Dis. 1973 Nov;32(6):557–564. doi: 10.1136/ard.32.6.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Wahl L. M., Wahl S. M., Mergenhagen S. E., Martin G. R. Collagenase production by lymphokine-activated macrophages. Science. 1975 Jan 24;187(4173):261–263. doi: 10.1126/science.163038. [DOI] [PubMed] [Google Scholar]
  43. Wegelius O., Klockars M., Vainio K. Collagenolytic activity in synovial fluid cells in rheumatoid arthritis. Ann Clin Res. 1970 Aug;21(2):171–174. [PubMed] [Google Scholar]
  44. Weissmann G. Lysosomal mechanisms of tissue injury in arthritis. N Engl J Med. 1972 Jan 20;286(3):141–147. doi: 10.1056/NEJM197201202860307. [DOI] [PubMed] [Google Scholar]
  45. Woolley D. E., Crossley M. J., Evanson J. M. Collagenase at sites of cartilage erosion in the rheumatoid joint. Arthritis Rheum. 1977 Jul-Aug;20(6):1231–1239. doi: 10.1002/art.1780200612. [DOI] [PubMed] [Google Scholar]
  46. Wright D. G., Malawista S. E. Mobilization and extracellular release of granular enzymes from human leukocytes during phagocytosis: inhibition by colchicine and cortisol but not by salicylate. Arthritis Rheum. 1973 Nov-Dec;16(6):749–758. doi: 10.1002/art.1780160608. [DOI] [PubMed] [Google Scholar]

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