Skip to main content
PMC home page
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1989 Oct;48(10):832–837. doi: 10.1136/ard.48.10.832

Interaction of polymorphonuclear leucocytes with patellar cartilage of immobilised arthritic joints: a scanning electron microscopic study.

P L van Lent 1, F H Wilms 1, W B van den Berg 1
PMCID: PMC1003890  PMID: 2818020

Abstract

Short term immobilisation (seven days) of an antigen induced arthritic knee joint of the mouse led to attachment of polymorphonuclear leucocytes to the cartilage surface and severe cartilage damage. The events at the patellar cartilage surface were studied by scanning electron microscopy and compared with the changes found in non-immobilised arthritic joints. At day 7 the immobilised arthritic joint showed an articular cartilage which was covered by a mat of fibrinous material and cell remnants. Moreover, inflammatory cells, clearly attached to the cartilage, showed large protrusions of their membranes. The cartilage surface, furthermore, showed numerous fissures of varying size and depth. These cartilage changes were much more pronounced in immobilised arthritic joints than in non-immobilised arthritic joints, and clear cell adhesion was exclusively found in the former. These findings show that immobilisation of inflamed mouse knee joints leads to increased damage and thus although clinical immobilisation is used to relieve pain, this may also risk damage to the articular cartilage.

Full text

PDF
835

Images in this article

833Image
on p.833
834Image
on p.834
834Image
on p.834
835Image
on p.835
835Image
on p.835
835Image
on p.835

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnaout M. A., Hakim R. M., Todd R. F., 3rd, Dana N., Colten H. R. Increased expression of an adhesion-promoting surface glycoprotein in the granulocytopenia of hemodialysis. N Engl J Med. 1985 Feb 21;312(8):457–462. doi: 10.1056/NEJM198502213120801. [DOI] [PubMed] [Google Scholar]
  2. Bates E. J., Kowanko I. C., Ferrante A. Conditioned medium from stimulated mononuclear leucocytes potentiates the ability of human neutrophils to damage human articular cartilage. Ann Rheum Dis. 1988 Jan;47(1):1–9. doi: 10.1136/ard.47.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cooke T. D., Sumia M., Elliott S., Maeda M. Immune complex mediated destruction of cartilage in antigen-induced arthritis of rabbits. J Rheumatol Suppl. 1983 Dec;11:103–107. [PubMed] [Google Scholar]
  4. Danon D., Goldstein L., Marikovsky Y., Skutelsky E. Use of cationized ferritin as a label of negative charges on cell surfaces. J Ultrastruct Res. 1972 Mar;38(5):500–510. doi: 10.1016/0022-5320(72)90087-1. [DOI] [PubMed] [Google Scholar]
  5. Keiser H., Greenwald R. A., Feinstein G., Janoff A. Degradation of cartilage proteoglycan by human leukocyte granule neutral proteases--a model of joint injury. II. Degradation of isolated bovine nasal cartilage proteoglycan. J Clin Invest. 1976 Mar;57(3):625–632. doi: 10.1172/JCI108318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Loermans H., Wirtz P. Inhibition of the expression of pathology in dystrophic mouse leg muscles by immobilization. Br J Exp Pathol. 1983 Apr;64(2):225–230. [PMC free article] [PubMed] [Google Scholar]
  7. McCormick J. R., Harkin M. M., Johnson K. J., Ward P. A. Suppression by superoxide dismutase of immune-complex--induced pulmonary alveolitis and dermal inflammation. Am J Pathol. 1981 Jan;102(1):55–61. [PMC free article] [PubMed] [Google Scholar]
  8. Mohr W., Wild A., Wolf H. P. Untersuchungen zur Pathogenese der entzündlichen Knorpelzerstörung bei der Adjuvansarthritis der Ratte. Z Rheumatol. 1980 Jul-Aug;39(7-8):212–222. [PubMed] [Google Scholar]
  9. Pettipher E. R., Henderson B., Moncada S., Higgs G. A. Leucocyte infiltration and cartilage proteoglycan loss in immune arthritis in the rabbit. Br J Pharmacol. 1988 Sep;95(1):169–176. doi: 10.1111/j.1476-5381.1988.tb16561.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schalkwijk J., van den Berg W. B., van de Putte L. B., Joosten L. A. Elastase secreted by activated polymorphonuclear leucocytes causes chondrocyte damage and matrix degradation in intact articular cartilage: escape from inactivation by alpha-1-proteinase inhibitor. Br J Exp Pathol. 1987 Feb;68(1):81–88. [PMC free article] [PubMed] [Google Scholar]
  11. Schalkwijk J., van den Berg W. B., van de Putte L. B., Joosten L. A. Hydrogen peroxide suppresses the proteoglycan synthesis of intact articular cartilage. J Rheumatol. 1985 Apr;12(2):205–210. [PubMed] [Google Scholar]
  12. Schalkwijk J., van den Berg W. B., van de Putte L. B., Joosten L. A., van der Sluis M. Effects of experimental joint inflammation on bone marrow and periarticular bone. A study of two types of arthritis, using variable degrees of inflammation. Br J Exp Pathol. 1985 Aug;66(4):435–444. [PMC free article] [PubMed] [Google Scholar]
  13. Ugai K., Ishikawa H., Hirohata K., Shirane H. Interaction of polymorphonuclear leukocytes with immune complexes trapped in rheumatoid articular cartilage. Arthritis Rheum. 1983 Dec;26(12):1434–1441. doi: 10.1002/art.1780261204. [DOI] [PubMed] [Google Scholar]
  14. Ugai K., Ziff M., Jasin H. E. Interaction of polymorphonuclear leukocytes with immune complexes trapped in joint collagenous tissues. Arthritis Rheum. 1979 Apr;22(4):353–364. doi: 10.1002/art.1780220407. [DOI] [PubMed] [Google Scholar]
  15. Woolley D. E., Crossley M. J., Evanson J. M. Collagenase at sites of cartilage erosion in the rheumatoid joint. Arthritis Rheum. 1977 Jul-Aug;20(6):1231–1239. doi: 10.1002/art.1780200612. [DOI] [PubMed] [Google Scholar]
  16. van Lent P. L., van den Berg W. B., Schalkwijk J., van de Putte L. B., van den Bersselaar L. Allergic arthritis induced by cationic antigens: relationship of chronicity with antigen retention and T-cell reactivity. Immunology. 1987 Oct;62(2):265–272. [PMC free article] [PubMed] [Google Scholar]
  17. van den Berg W. B., Kruijsen M. W., van de Putte L. B., van Beusekom H. J., van der Sluis-van der Pol M., Zwarts W. A. Antigen-induced and zymosan-induced arthritis in mice: studies on in vivo cartilage proteoglycan synthesis and chondrocyte death. Br J Exp Pathol. 1981 Jun;62(3):308–316. [PMC free article] [PubMed] [Google Scholar]
  18. van den Berg W. B., van de Putte L. B., Zwarts W. A., Joosten L. A. Electrical charge of the antigen determines intraarticular antigen handling and chronicity of arthritis in mice. J Clin Invest. 1984 Nov;74(5):1850–1859. doi: 10.1172/JCI111604. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES