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Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1990 Aug;49(8):611–614. doi: 10.1136/ard.49.8.611

Specific accumulation of cholesterol-rich liposomes in the inflammatory tissue of rats with adjuvant arthritis.

W G Love 1, N Amos 1, I W Kellaway 1, B D Williams 1
PMCID: PMC1004175  PMID: 2396866

Abstract

High performance liquid chromatography has shown that after intravenous injection cholesterol-poor liposomes (100 nm) are unstable and their phospholipid is redistributed. Under identical conditions cholesterol-rich liposomes remain structurally intact within the circulation. When injected intravenously cholesterol-rich liposomes accumulate within the inflamed paws of rats with adjuvant induced arthritis to the same extent as cholesterol-poor liposomes. Uptake in inflamed tissue of three cholesterol-rich liposome preparations was always significantly greater than the uptake noted in normal tissue. The degree of accumulation in inflamed tissue was found to depend on the size of the liposome, with the greatest uptake, 7% of the injected dose, achieved by the smallest vesicle (100 nm). These results indicate that intact liposomes accumulate at inflamed joint tissue sites. Therefore the passive targeting of anti-inflammatory drugs encapsulated within these liposomes could be contemplated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bangham A. D., Standish M. M., Watkins J. C. Diffusion of univalent ions across the lamellae of swollen phospholipids. J Mol Biol. 1965 Aug;13(1):238–252. doi: 10.1016/s0022-2836(65)80093-6. [DOI] [PubMed] [Google Scholar]
  2. Dave J., Patel H. M. Differentiation in hepatic and splenic phagocytic activity during reticuloendothelial blockade with cholesterol-free and cholesterol-rich liposomes. Biochim Biophys Acta. 1986 Sep 19;888(2):184–190. doi: 10.1016/0167-4889(86)90020-0. [DOI] [PubMed] [Google Scholar]
  3. GRAY S. J., STERLING K. The tagging of red cells and plasma proteins with radioactive chromium. J Clin Invest. 1950 Dec;29(12):1604–1613. doi: 10.1172/JCI102403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Johnson S. M. The inability of macrophages to digest liposomes containing a high proportion of cholesterol. Biochem Soc Trans. 1975;3(1):160–161. doi: 10.1042/bst0030160. [DOI] [PubMed] [Google Scholar]
  5. Kirby C., Clarke J., Gregoriadis G. Effect of the cholesterol content of small unilamellar liposomes on their stability in vivo and in vitro. Biochem J. 1980 Feb 15;186(2):591–598. doi: 10.1042/bj1860591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Love W. G., Amos N., Kellaway I. W., Williams B. D. Specific accumulation of technetium-99m radiolabelled, negative liposomes in the inflamed paws of rats with adjuvant induced arthritis: effect of liposome size. Ann Rheum Dis. 1989 Feb;48(2):143–148. doi: 10.1136/ard.48.2.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Love W. G., Amos N., Williams B. D., Kellaway I. W. High performance liquid chromatographic analysis of liposome stability. J Microencapsul. 1990 Jan-Mar;7(1):105–112. doi: 10.3109/02652049009028428. [DOI] [PubMed] [Google Scholar]
  8. Moghimi S. M., Patel H. M. Tissue specific opsonins for phagocytic cells and their different affinity for cholesterol-rich liposomes. FEBS Lett. 1988 Jun 6;233(1):143–147. doi: 10.1016/0014-5793(88)81372-3. [DOI] [PubMed] [Google Scholar]
  9. O'Sullivan M. M., Powell N., French A. P., Williams K. E., Morgan J. R., Williams B. D. Inflammatory joint disease: a comparison of liposome scanning, bone scanning, and radiography. Ann Rheum Dis. 1988 Jun;47(6):485–491. doi: 10.1136/ard.47.6.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PEARSON C. M. Development of arthritis, periarthritis and periostitis in rats given adjuvants. Proc Soc Exp Biol Med. 1956 Jan;91(1):95–101. doi: 10.3181/00379727-91-22179. [DOI] [PubMed] [Google Scholar]
  11. Papahadjopoulos D., Poste G., Schaeffer B. E. Fusion of mammalian cells by unilamellar lipid vesicles: inflluence of lipid surface charge, fluidity and cholesterol. Biochim Biophys Acta. 1973 Sep 27;323(1):23–42. doi: 10.1016/0005-2736(73)90429-x. [DOI] [PubMed] [Google Scholar]
  12. Patel H. M., Tuzel N. S., Ryman B. E. Inhibitory effect of cholesterol on the uptake of liposomes by liver and spleen. Biochim Biophys Acta. 1983 Dec 13;761(2):142–151. doi: 10.1016/0304-4165(83)90223-4. [DOI] [PubMed] [Google Scholar]
  13. Richardson V. J., Jeyasingh K., Jewkes R. F., Ryman B. E., Tattersall M. H. Properties of [99mTc] technetium-labelled liposomes in normal and tumour-bearing rats. Biochem Soc Trans. 1977;5(1):290–291. doi: 10.1042/bst0050290. [DOI] [PubMed] [Google Scholar]
  14. Roerdink F. H., Regts J., Handel T., Sullivan S. M., Baldeschwieler J. D., Scherphof G. L. Effect of cholesterol on the uptake and intracellular degradation of liposomes by liver and spleen; a combined biochemical and gamma-ray perturbed angular correlation study. Biochim Biophys Acta. 1989 Apr 14;980(2):234–240. doi: 10.1016/0005-2736(89)90404-5. [DOI] [PubMed] [Google Scholar]
  15. Scherphof G., Morselt H. On the size-dependent disintegration of small unilamellar phosphatidylcholine vesicles in rat plasma. Evidence of complete loss of vesicle structure. Biochem J. 1984 Jul 15;221(2):423–429. doi: 10.1042/bj2210423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Senior J., Gregoriadis G. Stability of small unilamellar liposomes in serum and clearance from the circulation: the effect of the phospholipid and cholesterol components. Life Sci. 1982 Jun 14;30(24):2123–2136. doi: 10.1016/0024-3205(82)90455-6. [DOI] [PubMed] [Google Scholar]
  17. Trentham D. E., Townes A. S., Kang A. H. Autoimmunity to type II collagen an experimental model of arthritis. J Exp Med. 1977 Sep 1;146(3):857–868. doi: 10.1084/jem.146.3.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wetterau J. R., Jonas A. Effect of dipalmitoylphosphatidylcholine vesicle curvature on the reaction with human apolipoprotein A-I. J Biol Chem. 1982 Sep 25;257(18):10961–10966. [PubMed] [Google Scholar]
  19. Williams B. D., O'Sullivan M. M., Saggu G. S., Williams K. E., Williams L. A., Morgan J. R. Synovial accumulation of technetium labelled liposomes in rheumatoid arthritis. Ann Rheum Dis. 1987 Apr;46(4):314–318. doi: 10.1136/ard.46.4.314. [DOI] [PMC free article] [PubMed] [Google Scholar]

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