Skip to main content
Annals of the Rheumatic Diseases logoLink to Annals of the Rheumatic Diseases
. 1993 Dec;52(12):891–894. doi: 10.1136/ard.52.12.891

Analysis of a T-cell receptor V beta segment implicated in susceptibility to rheumatoid arthritis: V beta 2 germline polymorphism does not encode susceptibility.

K Pile 1, P Wordsworth 1, F Lioté 1, T Bardin 1, J Bell 1, F Cornélis 1
PMCID: PMC1005222  PMID: 8311542

Abstract

OBJECTIVES--The assessment of allelic polymorphism of the T cell receptor gene segment, TCRBV2S1, in rheumatoid arthritis. METHODS--A total of 136 patients with rheumatoid arthritis (RA) (ACR criteria) and 150 controls were TCRBV2S1 genotyped using a nested PCR amplification strategy followed by single-strand conformation polymorphism (SSCP) analysis. RESULTS--The SSCP typing method detected two previously unknown alleles of the TCRBV2S1 gene segment. The TCRBV2S1 allele, genotype and inferred phenotype frequencies were similar in the RA patients and controls. No differences were apparent after the RA patients had been partitioned according to their HLA-DR genotypes. CONCLUSIONS--SSCP analysis is a rapid and efficient method of typing T cell receptor germline polymorphisms. Allelic polymorphism of the T cell receptor variable segment, TCRBV2S1, does not influence susceptibility to RA.

Full text

PDF
892

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acha-Orbea H., Mitchell D. J., Timmermann L., Wraith D. C., Tausch G. S., Waldor M. K., Zamvil S. S., McDevitt H. O., Steinman L. Limited heterogeneity of T cell receptors from lymphocytes mediating autoimmune encephalomyelitis allows specific immune intervention. Cell. 1988 Jul 15;54(2):263–273. doi: 10.1016/0092-8674(88)90558-2. [DOI] [PubMed] [Google Scholar]
  2. Bowness P., Bell J. T-cell receptors and rheumatic disease: approaches to repertoire analysis. Br J Rheumatol. 1992 Jan;31(1):3–8. doi: 10.1093/rheumatology/31.1.3. [DOI] [PubMed] [Google Scholar]
  3. Charmley P., Wang K., Hood L., Nickerson D. A. Identification and physical mapping of a polymorphic human T cell receptor V beta gene with a frequent null allele. J Exp Med. 1993 Jan 1;177(1):135–143. doi: 10.1084/jem.177.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cornélis F., Pile K., Loveridge J., Moss P., Harding R., Julier C., Bell J. Systematic study of human alpha beta T cell receptor V segments shows allelic variations resulting in a large number of distinct T cell receptor haplotypes. Eur J Immunol. 1993 Jun;23(6):1277–1283. doi: 10.1002/eji.1830230613. [DOI] [PubMed] [Google Scholar]
  5. Maksymowych W. P., Gabriel C. A., Luyrink L., Melin-Aldana H., Elma M., Giannini E. H., Lovell D. J., Van Kerckhove C., Leiden J., Choi E. Polymorphism in a T-cell receptor variable gene is associated with susceptibility to a juvenile rheumatoid arthritis subset. Immunogenetics. 1992;35(4):257–262. doi: 10.1007/BF00166831. [DOI] [PubMed] [Google Scholar]
  6. Marguerie C., Lunardi C., So A. PCR-based analysis of the TCR repertoire in human autoimmune diseases. Immunol Today. 1992 Sep;13(9):336–338. doi: 10.1016/0167-5699(92)90166-5. [DOI] [PubMed] [Google Scholar]
  7. Moss P. A., Rosenberg W. M., Bell J. I. The human T cell receptor in health and disease. Annu Rev Immunol. 1992;10:71–96. doi: 10.1146/annurev.iy.10.040192.000443. [DOI] [PubMed] [Google Scholar]
  8. Orita M., Suzuki Y., Sekiya T., Hayashi K. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics. 1989 Nov;5(4):874–879. doi: 10.1016/0888-7543(89)90129-8. [DOI] [PubMed] [Google Scholar]
  9. Reyburn H., Cornélis F., Russell V., Harding R., Moss P., Bell J. Allelic polymorphism of human T-cell receptor V alpha gene segments. Immunogenetics. 1993;38(4):287–291. doi: 10.1007/BF00188806. [DOI] [PubMed] [Google Scholar]
  10. Sewell K. L., Trentham D. E. Pathogenesis of rheumatoid arthritis. Lancet. 1993 Jan 30;341(8840):283–286. doi: 10.1016/0140-6736(93)92627-6. [DOI] [PubMed] [Google Scholar]
  11. Uematsu Y., Wege H., Straus A., Ott M., Bannwarth W., Lanchbury J., Panayi G., Steinmetz M. The T-cell-receptor repertoire in the synovial fluid of a patient with rheumatoid arthritis is polyclonal. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8534–8538. doi: 10.1073/pnas.88.19.8534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wordsworth B. P., Allsopp C. E., Young R. P., Bell J. I. HLA-DR typing using DNA amplification by the polymerase chain reaction and sequential hybridization to sequence-specific oligonucleotide probes. Immunogenetics. 1990;32(6):413–418. doi: 10.1007/BF00241635. [DOI] [PubMed] [Google Scholar]
  13. Wordsworth B. P., Bell J. I. The immunogenetics of rheumatoid arthritis. Springer Semin Immunopathol. 1992;14(1):59–78. doi: 10.1007/BF00197132. [DOI] [PubMed] [Google Scholar]
  14. van Eden W., Thole J. E., van der Zee R., Noordzij A., van Embden J. D., Hensen E. J., Cohen I. R. Cloning of the mycobacterial epitope recognized by T lymphocytes in adjuvant arthritis. Nature. 1988 Jan 14;331(6152):171–173. doi: 10.1038/331171a0. [DOI] [PubMed] [Google Scholar]

Articles from Annals of the Rheumatic Diseases are provided here courtesy of BMJ Publishing Group

RESOURCES