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. 1976 Jun;35(3):240–245. doi: 10.1136/ard.35.3.240

Detection of IgG rheumatoid factor by concanavalin A treatment and complement fixation with IgG rheumatoid factor.

K Tanimoto, T Moritoh, T Azuma, Y Horiuchi
PMCID: PMC1006547  PMID: 984904

Abstract

Concanavalin A (Con A) froms precipitates with carbohydrate-rich protein such as IgM, IgD, IgE, and IgA. Since IgG contains little carbohydrate and does not react with Con A, the activity of IgG-rheumatoid factor (RF) can be measured in the supernate of the Con A-treated serum. When the latex fixation test (LFT) and the sensitized sheep cell agglutination test (SSCA) were perfromed in the supernate for the detection of IgG-RF, LFT was positive in 32-1% of sera, out of 137 sera originally positive for LFT, and SSCA was positive in 18-5% of sera, out of 119 sera originally positive for SSCA. IgG-RF exhibited lower complement fixing ability than IgM-RF and correlated with agglutination titres of IgG-RF, while the CH50 of the original serum did not correlate with haemolytic activities of either IgM-RF or IgG-RF.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abruzzo J. L., Heimer R. The measurement of IgG anti-IgG antibody in rheumatoid arthritis. J Immunol Methods. 1974 Mar;4(2):149–160. doi: 10.1016/0022-1759(74)90057-x. [DOI] [PubMed] [Google Scholar]
  2. Adachi M., Atsumi T., Saito N., Nakamura M., Horiuchi Y. Detection of IgA- and IgG-rheumatoid factors by antiglobulin augmentation technique. Int Arch Allergy Appl Immunol. 1969;35(1):77–87. doi: 10.1159/000230161. [DOI] [PubMed] [Google Scholar]
  3. Bianco N. E., Panush R. S., Stillman J. S., Schur P. H. Immunologic studies of juvenile rheumatoid arthritis. Arthritis Rheum. 1971 Nov-Dec;14(6):685–696. doi: 10.1002/art.1780140603. [DOI] [PubMed] [Google Scholar]
  4. CHODIRKER W. B., TOMASI T. B., Jr Low-molecular-weight rheumatoid factor. J Clin Invest. 1963 Jun;42:876–884. doi: 10.1172/JCI104780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Estes D., Atra E., Peltier A. Immunofluorescent method for the detection of antigammaglobulin antibodies. Arthritis Rheum. 1973 Jan-Feb;16(1):59–65. doi: 10.1002/art.1780160109. [DOI] [PubMed] [Google Scholar]
  6. Franco A. E., Schur P. H. Hypocomplementemia in rheumatoid arthritis. Arthritis Rheum. 1971 Mar-Apr;14(2):231–238. doi: 10.1002/art.1780140206. [DOI] [PubMed] [Google Scholar]
  7. Goldstein I. J., So L. L., Yang Y., Callies Q. C. Protein-carbohydrate interaction. XIX. The interaction of concanavalin A with IgM and the glycoprotein phytohemagglutinins of the waxbean and the soybean. J Immunol. 1969 Oct;103(4):695–698. [PubMed] [Google Scholar]
  8. Hannestad K. Presence of aggregated gamma-G-globulin in certain rheumatoid synovial effusions. Clin Exp Immunol. 1967 Jul;2(4):511–529. [PMC free article] [PubMed] [Google Scholar]
  9. Heimer R., Levin F. M. On the distribution of rheumatoid factors among the immunoglobulins. Immunochemistry. 1966 Jan;3(1):1–10. doi: 10.1016/0019-2791(66)90276-x. [DOI] [PubMed] [Google Scholar]
  10. Leon M. A. Concanavalin A reaction with human normal immunoglobulin G and myeloma immunoglobulin G. Science. 1967 Dec 8;158(3806):1325–1326. doi: 10.1126/science.158.3806.1325. [DOI] [PubMed] [Google Scholar]
  11. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  12. Marcus R. L., Townes A. S. The occurrence of cryoproteins in synovial fluid; the association of a complement-fixing activity in rheumatoid synovial fluid with cold-precipitable protein. J Clin Invest. 1971 Feb;50(2):282–293. doi: 10.1172/JCI106493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mellbye O. J., Natvig J. B. Evidence for immune complexes containing antibody to the pepsin site of IgG in rheumatoid synovial fluids. Clin Exp Immunol. 1971 Jun;8(6):889–899. [PMC free article] [PubMed] [Google Scholar]
  14. Mongan E. S., Cass R. M., Jacox R. F., Vaughen J. H. A study of the relation of seronegative and seropositive rheumatoid arthritis to each other and to necrotizing vasculitis. Am J Med. 1969 Jul;47(1):23–35. doi: 10.1016/0002-9343(69)90238-1. [DOI] [PubMed] [Google Scholar]
  15. Munthe E., Natvig J. B. Characterization of IgG complexes in eluates from rheumatoid tissue. Clin Exp Immunol. 1971 Feb;8(2):249–262. [PMC free article] [PubMed] [Google Scholar]
  16. Panush R. S., Bianco N. E., Schur P. H. Serum and synovial fluid IgG, IgA and IgM antigammaglobulins in rheumatoid arthritis. Arthritis Rheum. 1971 Nov-Dec;14(6):737–747. doi: 10.1002/art.1780140609. [DOI] [PubMed] [Google Scholar]
  17. Tanimoto K., Cooper N. R., Johnson J. S., Vaughan J. H. Complement fixation by rheumatoid factor. J Clin Invest. 1975 Mar;55(3):437–445. doi: 10.1172/JCI107949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Theofilopoulos A. N., Burtonboy G., LoSpalluto J. J., Ziff M. IgM rheumatoid factor and low molecular weight IgM. An association with vasculitis. Arthritis Rheum. 1974 May-Jun;17(3):272–284. doi: 10.1002/art.1780170312. [DOI] [PubMed] [Google Scholar]
  19. Torrigiani G., Roitt I. M. Antiglobulin factors in sera from patients with rheumatoid arthritis and normal subjects. Quantitative estimation in different immunoglobulin classes. Ann Rheum Dis. 1967 Jul;26(4):334–340. doi: 10.1136/ard.26.4.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Townes A. S., Marcus R. L. Complement-fixing activity of rheumatoid synovial fluid. Ann Rheum Dis. 1972 Sep;31(5):393–401. doi: 10.1136/ard.31.5.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weinstein Y., Givol D., Strausbauch P. H. The fractionation of immunoglobulins with insolubilized concanavalin A. J Immunol. 1972 Dec;109(6):1402–1404. [PubMed] [Google Scholar]
  22. Winchester R. J., Agnello V., Kunkel H. G. The joint-fluid gammaG-globulin complexes and their relationship to intraarticular complement dimunition. Ann N Y Acad Sci. 1969 Dec 10;168(1):195–203. doi: 10.1111/j.1749-6632.1969.tb43108.x. [DOI] [PubMed] [Google Scholar]

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