Abstract
Beck, E. G., Holt, P. F., and Nasrallah, E. T. (1971).Brit. J. industr. Med.,28, 179-185. Effects of chrysotile and acid-treated chrysotile on macrophage cultures. The addition of chrysotile asbestos to monolayer cultures of peritoneal and alveolar macrophages produces an increase in membrane permeability, as measured by eosin uptake and lactic dehydrogenase activity of the supernatant fluid. The lactate synthesis is increased, however. It is suggested that the permeability of the cell membrane is increased while dust particles are being phagocytosed, which may take several hours when the particles are fibrous, but that this does not imply cell damage.
Treatment of chrysotile with acid, which leaves a silica surface, results in a product that reduces lactate synthesis, implying cytotoxicity. This change is counteracted by poly(2-vinyl-pyridine 1-oxide). The polymer does not affect the properties of the native chrysotile.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BECK E. G., BRUCH J., BROCKHAUS A. [Alteration of the cytotoxic effect of quartz on mouse fibroblasts (strain L) by polyvinylpyridine-N-oxide]. Z Zellforsch Mikrosk Anat. 1963;59:568–576. [PubMed] [Google Scholar]
- BRUNS F. H., HORN H. D. Quantitative Bestimmung von L(+)-Milchsäure mit Milchsäuredehydrogenase. Biochim Biophys Acta. 1956 Aug;21(2):378–380. doi: 10.1016/0006-3002(56)90023-3. [DOI] [PubMed] [Google Scholar]
- Botham S. K., Holt P. F. The mechanism of formation of asbestos bodies. J Pathol Bacteriol. 1968 Oct;96(2):443–453. doi: 10.1002/path.1700960223. [DOI] [PubMed] [Google Scholar]
- HANKS J. H., WALLACE J. H. Determination of cell viability. Proc Soc Exp Biol Med. 1958 May;98(1):188–192. doi: 10.3181/00379727-98-23985. [DOI] [PubMed] [Google Scholar]
- HOLT P. F., MILLS J., YOUNG D. K. THE EARLY EFFECTS OF CHRYSOTILE ASBESTOS DUST ON THE RAT LUNG. J Pathol Bacteriol. 1964 Jan;87:15–23. doi: 10.1002/path.1700870103. [DOI] [PubMed] [Google Scholar]
- Parazzi E., Pernis B., Secchi G. C., Vigliani E. C. Studies on "in vitro" cytotoxicity of asbestos dusts. Med Lav. 1968 Oct;59(10):561–576. [PubMed] [Google Scholar]
- Pernis B., Vigliani E. C., Marchisio M. A., Zanardi S. Observations on the effect of asbestos on cells "in vitro". Med Lav. 1966 Dec;57(12):721–729. [PubMed] [Google Scholar]
- WIELAND T., PFLEIDERER G. Nachweis der Heterogenität von Milchsäure-dehydrogenasen verschiedenen Ursprungs durch Trägerelektrophorese. Biochem Z. 1957;329(2):112–116. [PubMed] [Google Scholar]