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. 1995 Aug;54(8):678–680. doi: 10.1136/ard.54.8.678

HLA class II antigens associated with systemic lupus erythematosus in black South Africans.

M Rudwaleit 1, M Tikly 1, K Gibson 1, K Pile 1, P Wordsworth 1
PMCID: PMC1009967  PMID: 7677446

Abstract

OBJECTIVE--To assess the associations of HLA class II antigens with systemic lupus erythematosus (SLE) in black South Africans. METHODS--HLA-DRB1 genotype frequencies assigned by polymerase chain reaction (PCR) amplification and sequence specific oligonucleotide probes were compared between 49 black SLE patients from Baragwanath Hospital and 87 ethnically matched controls. HLA-DQA1 and -DQB1 genotypes were also assigned in 45 of the SLE patients and 74 controls by PCR using sequence specific primers. RESULTS--HLA-DRB1*02 was increased in the patients compared with controls (odds ratio = 3.67; 95% confidence interval = 1.49 to 9.02; p < 0.005). HLA-DQB1*0201 was not associated with development of the disease itself, but was associated with the presence of Ro antibodies (p = 0.01). HLA-DRB1*03 was less strongly linked to DQB1*02 in this population than in white populations and was not associated with SLE. CONCLUSIONS--In black South Africans there is evidence for a locus on DR2 haplotypes contributing to SLE. Another gene, possibly HLA-DQB1*02, not linked to DR2 is involved in the subset of patients exhibiting Ro antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alarif L. I., Ruppert G. B., Wilson R., Jr, Barth W. F. HLA-DR antigens in Blacks with rheumatoid arthritis and systemic lupus erythematosus. J Rheumatol. 1983 Apr;10(2):297–300. [PubMed] [Google Scholar]
  2. Bunce M., Taylor C. J., Welsh K. I. Rapid HLA-DQB typing by eight polymerase chain reaction amplifications with sequence-specific primers (PCR-SSP). Hum Immunol. 1993 Aug;37(4):201–206. doi: 10.1016/0198-8859(93)90502-r. [DOI] [PubMed] [Google Scholar]
  3. Davies E. J., Hillarby M. C., Cooper R. G., Hay E. M., Green J. R., Shah S., Bernstein R. M., Holt P. J., Grennan D. M. HLA-DQ, DR and complement C4 variants in systemic lupus erythematosus. Br J Rheumatol. 1993 Oct;32(10):870–875. doi: 10.1093/rheumatology/32.10.870. [DOI] [PubMed] [Google Scholar]
  4. Deapen D., Escalante A., Weinrib L., Horwitz D., Bachman B., Roy-Burman P., Walker A., Mack T. M. A revised estimate of twin concordance in systemic lupus erythematosus. Arthritis Rheum. 1992 Mar;35(3):311–318. doi: 10.1002/art.1780350310. [DOI] [PubMed] [Google Scholar]
  5. Doherty D. G., Ireland R., Demaine A. G., Wang F., Veerapan K., Welsh K. I., Vergani D. Major histocompatibility complex genes and susceptibility to systemic lupus erythematosus in southern Chinese. Arthritis Rheum. 1992 Jun;35(6):641–646. doi: 10.1002/art.1780350607. [DOI] [PubMed] [Google Scholar]
  6. Green J. R., Chiew M. K., Low H. C., Woodrow J. C. The association between HLA antigens and the presence of certain diseases. Stat Med. 1983 Jan-Mar;2(1):79–85. doi: 10.1002/sim.4780020109. [DOI] [PubMed] [Google Scholar]
  7. Hartung K., Baur M. P., Coldewey R., Fricke M., Kalden J. R., Lakomek H. J., Peter H. H., Schendel D., Schneider P. M., Seuchter S. A. Major histocompatibility complex haplotypes and complement C4 alleles in systemic lupus erythematosus. Results of a multicenter study. J Clin Invest. 1992 Oct;90(4):1346–1351. doi: 10.1172/JCI116000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Howard P. F., Hochberg M. C., Bias W. B., Arnett F. C., Jr, McLean R. H. Relationship between C4 null genes, HLA-D region antigens, and genetic susceptibility to systemic lupus erythematosus in Caucasian and black Americans. Am J Med. 1986 Aug;81(2):187–193. doi: 10.1016/0002-9343(86)90250-0. [DOI] [PubMed] [Google Scholar]
  9. Kachru R. B., Sequeira W., Mittal K. K., Siegel M. E., Telischi M. A significant increase of HLA-DR3 and DR2 in systemic lupus erythematosus among blacks. J Rheumatol. 1984 Aug;11(4):471–474. [PubMed] [Google Scholar]
  10. Olerup O., Aldener A., Fogdell A. HLA-DQB1 and -DQA1 typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours. Tissue Antigens. 1993 Mar;41(3):119–134. doi: 10.1111/j.1399-0039.1993.tb01991.x. [DOI] [PubMed] [Google Scholar]
  11. Olsen M. L., Goldstein R., Arnett F. C., Duvic M., Pollack M., Reveille J. D. C4A gene deletion and HLA associations in black Americans with systemic lupus erythematosus. Immunogenetics. 1989;30(1):27–33. doi: 10.1007/BF02421466. [DOI] [PubMed] [Google Scholar]
  12. Reveille J. D., Schrohenloher R. E., Acton R. T., Barger B. O. DNA analysis of HLA-DR and DQ genes in American blacks with systemic lupus erythematosus. Arthritis Rheum. 1989 Oct;32(10):1243–1251. doi: 10.1002/anr.1780321009. [DOI] [PubMed] [Google Scholar]
  13. Wilson W. A., Scopelitis E., Michalski J. P. Association of HLA-DR7 with both antibody to SSA(Ro) and disease susceptibility in blacks with systemic lupus erythematosus. J Rheumatol. 1984 Oct;11(5):653–657. [PubMed] [Google Scholar]
  14. Wordsworth B. P., Allsopp C. E., Young R. P., Bell J. I. HLA-DR typing using DNA amplification by the polymerase chain reaction and sequential hybridization to sequence-specific oligonucleotide probes. Immunogenetics. 1990;32(6):413–418. doi: 10.1007/BF00241635. [DOI] [PubMed] [Google Scholar]

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