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. 1999 May;52(5):367–371. doi: 10.1136/jcp.52.5.367

Correlation between epithelial cell proliferation and histological grading in gastric mucosa.

D A Lynch 1, N P Mapstone 1, A M Clarke 1, P Jackson 1, P Moayyedi 1, M F Dixon 1, P Quirke 1, A T Axon 1
PMCID: PMC1023074  PMID: 10560358

Abstract

AIM: To determine if there is a correlation between the histological findings in the gastric mucosa and the degree of cell proliferation in gastric antral biopsies. METHODS: Cell proliferation in gastric antral biopsies was determined by in vitro bromodeoxyuridine labelling. Histological sections were assessed using the Sydney System. RESULTS: There was a positive correlation between antral mucosal cell proliferation and the acute inflammatory cell infiltrate (r = 0.29; p = 0.03). There was a stronger correlation with the chronic inflammatory cell infiltrate (r = 0.53; p < 0.0001) and the density of H pylori colonisation (r = 0.54; p < 0.0001). There was no correlation between gastric epithelial proliferation and the degree of atrophy. Stepwise multiple regression indicates that the only independent predictor of epithelial cell proliferation is the density of H pylori colonisation (p < 0.0001). CONCLUSIONS: H pylori increases gastric epithelial cell proliferation through the mucosal inflammatory response and probably by other means. The strong correlation between epithelial proliferation, the chronic inflammatory cell infiltrate, and the density of H pylori colonization may have implications for gastric carcinogenesis.

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Selected References

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  1. Bechi P., Balzi M., Becciolini A., Amorosi A., Scubla E., Giachè V., Mazzanti R., Tonelli P., Cortesini C. Gastric cell proliferation kinetics and bile reflux after partial gastrectomy. Am J Gastroenterol. 1991 Oct;86(10):1424–1432. [PubMed] [Google Scholar]
  2. Cahill R. J., Kilgallen C., Beattie S., Hamilton H., O'Morain C. Gastric epithelial cell kinetics in the progression from normal mucosa to gastric carcinoma. Gut. 1996 Feb;38(2):177–181. doi: 10.1136/gut.38.2.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Charnley G., Tannenbaum S. R. Flow cytometric analysis of the effect of sodium chloride on gastric cancer risk in the rat. Cancer Res. 1985 Nov;45(11 Pt 2):5608–5616. [PubMed] [Google Scholar]
  4. Deschner E. E., Winawer S. J., Lipkin M. Patterns of nucleic acid and protein synthesis in normal human gastric mucosa and atrophic gastritis. J Natl Cancer Inst. 1972 Jun;48(6):1567–1574. [PubMed] [Google Scholar]
  5. Di Marco E., Pierce J. H., Aaronson S. A., Di Fiore P. P. Mechanisms by which EGF receptor and TGF alpha contribute to malignant transformation. Nat Immun Cell Growth Regul. 1990;9(3):209–221. [PubMed] [Google Scholar]
  6. Emery P., Salmon M. The immune response. 2. Systemic mediators of inflammation. Br J Hosp Med. 1991 Mar;45(3):164–168. [PubMed] [Google Scholar]
  7. Fan X. G., Kelleher D., Fan X. J., Xia H. X., Keeling P. W. Helicobacter pylori increases proliferation of gastric epithelial cells. Gut. 1996 Jan;38(1):19–22. doi: 10.1136/gut.38.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Filipe M. I., Potet F., Bogomoletz W. V., Dawson P. A., Fabiani B., Chauveinc P., Fenzy A., Gazzard B., Goldfain D., Zeegen R. Incomplete sulphomucin-secreting intestinal metaplasia for gastric cancer. Preliminary data from a prospective study from three centres. Gut. 1985 Dec;26(12):1319–1326. doi: 10.1136/gut.26.12.1319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haenszel W., Kurihara M., Segi M., Lee R. K. Stomach cancer among Japanese in Hawaii. J Natl Cancer Inst. 1972 Oct;49(4):969–988. [PubMed] [Google Scholar]
  10. Hansen O. H., Johansen A., Larsen J. K., Pedersen T., Svendsen L. B. Relationship between gastric acid secretion, histopathology, and cell proliferation kinetics in human gastric mucosa. Gastroenterology. 1977 Sep;73(3):453–456. [PubMed] [Google Scholar]
  11. Hansen O. H., Pedersen T., Larsen J. K. Cell proliferation kinetics in normal human gastric mucosa. Studies on diurnal fluctuations and effect of food ingestion. Gastroenterology. 1976 Jun;70(6):1051–1054. [PubMed] [Google Scholar]
  12. Hart-Hansen O., Johansen A., Larsen J. K., Svendsen L. B. Cell proliferation in normal and diseased gastric mucosa. Autoradiography after in vitro continuous labelling with tritiated thymidine. Acta Pathol Microbiol Scand A. 1979 May;87A(3):217–222. [PubMed] [Google Scholar]
  13. Hoey J., Montvernay C., Lambert R. Wine and tobacco: risk factors for gastric cancer in France. Am J Epidemiol. 1981 Jun;113(6):668–674. doi: 10.1093/oxfordjournals.aje.a113146. [DOI] [PubMed] [Google Scholar]
  14. Jankowski J., al-Rawi H. J., Johnston D. A., Hopwood D., Filipe M. I., Coghill G., Wormsley K. G. Growth regulatory peptides in gastric mucosa. Clin Sci (Lond) 1992 May;82(5):581–587. doi: 10.1042/cs0820581. [DOI] [PubMed] [Google Scholar]
  15. Jass J. R., Filipe M. I. A variant of intestinal metaplasia associated with gastric carcinoma: a histochemical study. Histopathology. 1979 May;3(3):191–199. doi: 10.1111/j.1365-2559.1979.tb02996.x. [DOI] [PubMed] [Google Scholar]
  16. Jass J. R. Role of intestinal metaplasia in the histogenesis of gastric carcinoma. J Clin Pathol. 1980 Sep;33(9):801–810. doi: 10.1136/jcp.33.9.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Katelaris P. H., Seow F., Lin B. P., Napoli J., Ngu M. C., Jones D. B. Effect of age, Helicobacter pylori infection, and gastritis with atrophy on serum gastrin and gastric acid secretion in healthy men. Gut. 1993 Aug;34(8):1032–1037. doi: 10.1136/gut.34.8.1032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lunec J. Free radicals: their involvement in disease processes. Ann Clin Biochem. 1990 May;27(Pt 3):173–182. doi: 10.1177/000456329002700301. [DOI] [PubMed] [Google Scholar]
  19. Lynch D. A., Mapstone N. P., Clarke A. M., Jackson P., Dixon M. F., Quirke P., Axon A. T. Cell proliferation in the gastric corpus in Helicobacter pylori associated gastritis and after gastric resection. Gut. 1995 Mar;36(3):351–353. doi: 10.1136/gut.36.3.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lynch D. A., Mapstone N. P., Clarke A. M., Sobala G. M., Jackson P., Morrison L., Dixon M. F., Quirke P., Axon A. T. Cell proliferation in Helicobacter pylori associated gastritis and the effect of eradication therapy. Gut. 1995 Mar;36(3):346–350. doi: 10.1136/gut.36.3.346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Marshall B. J., Langton S. R. Urea hydrolysis in patients with Campylobacter pyloridis infection. Lancet. 1986 Apr 26;1(8487):965–966. doi: 10.1016/s0140-6736(86)91060-3. [DOI] [PubMed] [Google Scholar]
  22. Meyer M. B., Luk G. D., Sotelo J. M., Cohen B. H., Menkes H. A. Hypothesis: the role of the lung in stomach carcinogenesis. Am Rev Respir Dis. 1980 May;121(5):887–892. doi: 10.1164/arrd.1980.121.5.887. [DOI] [PubMed] [Google Scholar]
  23. Pagnini C. A., Farini R., Cardin F., Di Mario F., Rugge M., Naccarato R. Gastric epithelial dysplasia and sulphomucin-type intestinal metaplasia. Tumori. 1983 Aug 31;69(4):355–357. doi: 10.1177/030089168306900414. [DOI] [PubMed] [Google Scholar]
  24. Rauws E. A., Langenberg W., Houthoff H. J., Zanen H. C., Tytgat G. N. Campylobacter pyloridis-associated chronic active antral gastritis. A prospective study of its prevalence and the effects of antibacterial and antiulcer treatment. Gastroenterology. 1988 Jan;94(1):33–40. [PubMed] [Google Scholar]
  25. Silva S., Filipe M. I., Pinho A. Variants of intestinal metaplasia in the evolution of chronic atrophic gastritis and gastric ulcer. A follow up study. Gut. 1990 Oct;31(10):1097–1104. doi: 10.1136/gut.31.10.1097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sipponen P., Seppälä K., Varis K., Hjelt L., Ihamäki T., Kekki M., Siurala M. Intestinal metaplasia with colonic-type sulphomucins in the gastric mucosa; its association with gastric carcinoma. Acta Pathol Microbiol Scand A. 1980 Jul;88(4):217–224. doi: 10.1111/j.1699-0463.1980.tb02489.x. [DOI] [PubMed] [Google Scholar]
  27. Wagner S., Beil W., Westermann J., Logan R. P., Bock C. T., Trautwein C., Bleck J. S., Manns M. P. Regulation of gastric epithelial cell growth by Helicobacter pylori: offdence for a major role of apoptosis. Gastroenterology. 1997 Dec;113(6):1836–1847. doi: 10.1016/s0016-5085(97)70003-9. [DOI] [PubMed] [Google Scholar]

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