Skip to main content
PMC home page
The British Journal of Ophthalmology logoLink to The British Journal of Ophthalmology
. 1979 Aug;63(8):551–559. doi: 10.1136/bjo.63.8.551

A scanning electron microscopic examination of retinoblastoma in tissue culture.

H R Taylor, N Carroll, I Jack, G W Crock
PMCID: PMC1043547  PMID: 476031

Abstract

Tissue cultures were established from 6 retinoblastomas and the cultured cells examined by scanning electron microscopy. Cells from cultures of normal fetal retinal and glial tissue were also examined by SEM. Though a number of different cell types were found to coexist in the retinoblastoma cultures, most had the features of cells of either glial or neuronal origin. Evidence of further differentiation of cells in each series was seen. These findings indicate that the cell of origin in retinoblastoma is a multipotential stem cell which, though neoplastic, is still capable of differentiation into both glial and neuronal series.

Full text

PDF
555

Images in this article

554Image
on p.554
554Image
on p.554
555Image
on p.555
555Image
on p.555
555Image
on p.555
555Image
on p.555
556Image
on p.556
556Image
on p.556
557Image
on p.557
557Image
on p.557

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albert D. M., Lahav M., Lesser R., Craft J. Recent observations regarding retinoblastoma. I. Ultrastructure, tissue culture growth, incidence, and animal models. Trans Ophthalmol Soc U K. 1974;94(4):909–928. [PubMed] [Google Scholar]
  2. Albert D. M., Tso M. O., Rabson A. S. In vitro growth of pure cultures of retinal pigment epithelium. Arch Ophthalmol. 1972 Jul;88(1):63–69. doi: 10.1001/archopht.1972.01000030065014. [DOI] [PubMed] [Google Scholar]
  3. Ben-Shaul Y., Moscona A. A. Scanning electron microscopy of embryonic neural retina cell surfaces. Dev Biol. 1975 Jun;44(2):386–393. doi: 10.1016/0012-1606(75)90410-8. [DOI] [PubMed] [Google Scholar]
  4. Craft J. L., Robinson N. L., Roth N. A., Albert D. M. Scanning electron microscopy of retinoblastoma. Exp Eye Res. 1978 Nov;27(5):519–531. doi: 10.1016/0014-4835(78)90137-9. [DOI] [PubMed] [Google Scholar]
  5. Dickson D. H., Ramsey M. S., Tonus J. G. Synapse formation in retinoblastoma tumours. Br J Ophthalmol. 1976 May;60(5):371–375. doi: 10.1136/bjo.60.5.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elul R., Brons J., Kravitz K. Surface charge modifications associated with proliferation and differentiation in neuroblastoma cultures. Nature. 1975 Dec 18;258(5536):616–617. doi: 10.1038/258616a0. [DOI] [PubMed] [Google Scholar]
  7. Gospodarowicz D., Mescher A. L., Birdwell C. R. Stimulation of corneal endothelial cell proliferations in vitro by fibroblast and epidermal growth factors. Exp Eye Res. 1977 Jul;25(1):75–89. doi: 10.1016/0014-4835(77)90248-2. [DOI] [PubMed] [Google Scholar]
  8. Hild W., Callas G. The behavior of retinal tissue in vitro, light and electron microscopic observations. Z Zellforsch Mikrosk Anat. 1967;80(1):1–21. doi: 10.1007/BF00331473. [DOI] [PubMed] [Google Scholar]
  9. Huang L. H., Sery T. W., Chen M. M., Cheung A. S., Keeney A. H. Experimental retinoblastoma. I. Morphology and behavior of cells cultivated in vitro. Am J Ophthalmol. 1970 Nov;70(5):771–777. [PubMed] [Google Scholar]
  10. LISS L., WOLTER J. R. Human retinal neurons in tissue culture. Am J Ophthalmol. 1961 Nov;52:834–841. doi: 10.1016/0002-9394(61)90909-6. [DOI] [PubMed] [Google Scholar]
  11. Lodin Z., Faltin J., Booher J., Hartman J. Differentiation of neurones and glial cells in cultures of dissociated dorsal root ganglia an electron-microscopic and scanning electron-microscopic study. Physiol Bohemoslov. 1973;22(2):109–114. [PubMed] [Google Scholar]
  12. POMERAT C. M., LITTLEJOHN L., Jr Observations on tissue culture of the human eye. South Med J. 1956 Mar;49(3):230–237. doi: 10.1097/00007611-195603000-00006. [DOI] [PubMed] [Google Scholar]
  13. Popoff N. A., Ellsworth R. M. The fine structure of retinoblastoma. In vivo and in vitro observations. Lab Invest. 1971 Nov;25(5):389–402. [PubMed] [Google Scholar]
  14. Prop F. J., Prop-Arnold G. C., Eijgenstein L. H. Gewebezüchtungsuntersuchungen zur Unterscheidung des erblichen vom sporadischen Retinoblastom. Klin Monbl Augenheilkd. 1975 Sep;167(3):396–404. [PubMed] [Google Scholar]
  15. Reid T. W., Albert D. M., Rabson A. S., Russell P., Craft J., Chu E. W., Tralka T. S., Wilcox J. L. Characteristics of an established cell line of retinoblastoma. J Natl Cancer Inst. 1974 Aug;53(2):347–360. doi: 10.1093/jnci/53.2.347. [DOI] [PubMed] [Google Scholar]
  16. Shein H. M. Propagation of human fetal spongioblasts and astrocytes in dispersed cell cultures. Exp Cell Res. 1965 Dec;40(3):554–569. doi: 10.1016/0014-4827(65)90234-x. [DOI] [PubMed] [Google Scholar]
  17. Ts'o M. O., Fine B. S., Zimmerman L. E. The nature of retinoblastoma. II. Photoreceptor differentiation: an electron microscopic study. Am J Ophthalmol. 1970 Mar;69(3):350–359. doi: 10.1016/0002-9394(70)92264-6. [DOI] [PubMed] [Google Scholar]
  18. Watters W. B., Buck R. C. An improved simple method of specimen preparation for replicas or scanning electron microscopy. J Microsc. 1971 Oct;94(2):185–187. doi: 10.1111/j.1365-2818.1971.tb03703.x. [DOI] [PubMed] [Google Scholar]
  19. YONEDA C., VAN HERICK W. Tissue culture cell strain derived from retinoblastoma. Am J Ophthalmol. 1963 May;55:987–992. [PubMed] [Google Scholar]

Articles from The British Journal of Ophthalmology are provided here courtesy of BMJ Publishing Group

RESOURCES