Skip to main content
The British Journal of Ophthalmology logoLink to The British Journal of Ophthalmology
. 1980 Sep;64(9):700–704. doi: 10.1136/bjo.64.9.700

Quantitative tear lysozyme assay: a new technique for transporting specimens.

D V Seal, I A Mackie, R L Coakes, B Farooqi
PMCID: PMC1043798  PMID: 7426595

Abstract

We have developed a method for assaying the concentration of tear lysozyme using eluates of tear fluid collected on filter paper discs. Specimens can be stored and transported to remote laboratories for assay. We have shown that the 'indirect' or eluate method gives statistically comparable results to the 'direct' method using fresh, neat tear fluid.

Full text

PDF
701

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ERICKSON O. F., HATLEN R., BERG M. Industrial tear study; filter-paper electrophoresis of tears, with results of an industrial study from 1,000 specimens sent by mail. Am J Ophthalmol. 1959 Jan;47(1 Pt 2):499–509. [PubMed] [Google Scholar]
  2. Horwitz B. L., Christensen G. R., Ritzmann S. R. Diurnal profiles of tear lysozyme and gamma A globulin. Ann Ophthalmol. 1978 Jan;10(1):75–80. [PubMed] [Google Scholar]
  3. Hypher T. J. Fluid uptake in Schirmer papers and its relevance to their employment in tear collection for lysozyme tests. Br J Ophthalmol. 1980 Sep;64(9):696–699. doi: 10.1136/bjo.64.9.696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hypher T. J. Uptake and loss of tears from filter paper discs employed in lysozyme tests. Br J Ophthalmol. 1979 Apr;63(4):251–255. doi: 10.1136/bjo.63.4.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mackie I. A., Seal D. V., Pescod J. M. Beta-adrenergic receptor blocking drugs: tear lysozyme and immunological screening for adverse reaction. Br J Ophthalmol. 1977 May;61(5):354–359. doi: 10.1136/bjo.61.5.354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mackie I. A., Seal D. V. Quantitative tear lysozyme assay in units of activity per microlitre. Br J Ophthalmol. 1976 Jan;60(1):70–74. doi: 10.1136/bjo.60.1.70. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The British Journal of Ophthalmology are provided here courtesy of BMJ Publishing Group

RESOURCES