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. 1976 Dec;52(6):404–408. doi: 10.1136/sti.52.6.404

Sensitivity of Trichomonas vaginalis to metronidazole, tinidazole, and nifuratel in vitro.

B Korner, H K Jensen
PMCID: PMC1045320  PMID: 1087577

Abstract

Prompted by the sensitivity of trichomonads to metronidazole and nifuratel in clinical practice, a study was conducted in 1971-1972 of 63 consecutive strains of Trichomonas vaginalis isolated from women with clinically refractory vaginal discharge. Their susceptibility to metronidazole, tinidazole, and nifuratel was tested, using a serial tube dilution technique. The minimum concentrations which in 48 hrs caused immobilization and lysis of trichomonads cultured in Diamond's medium was assessed. No differences in drug potency could be determined. The median trichomonistatic and trichomonicidal concentrations were 0-1 and 0-6 mug/ml. respectively when using an inoculum of 10,000 organisms per ml. An inoculum of 100,000 per ml. resulted in inhibitory concentrations of 1-0 and killing concentrations of 3-3 mug./ml. These levels are readily attained in blood and vaginal tissue after oral ingestion of the two imidazole derivatives. Thus, metronidazole has maintained its efficacy since it was first introduced more than a decade ago. The few therapeutic failures with metronidazole and tinidazole are considered to have been caused by pharmacokinetic deficiencies in the patients, or by re-infection.

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Selected References

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  1. Arnold M. Beobachtungen und Probleme bei der Behandlung der Trichomonas vaginalis. Ther Umsch. 1966 Sep;23(9):356–359. [PubMed] [Google Scholar]
  2. Aure J. C., Gjonnaess H. Metronidazole treatment of trichomonal vaginitis. A comparison of cure rates in 1961 and 1967. Acta Obstet Gynecol Scand. 1969;48(3):440–445. doi: 10.3109/00016346909156659. [DOI] [PubMed] [Google Scholar]
  3. BUSHBY S. R., COPP F. C. The antitrichomonal activity of amidonitrothiazoles. J Pharm Pharmacol. 1955 Feb;7(2):112–117. doi: 10.1111/j.2042-7158.1955.tb12014.x. [DOI] [PubMed] [Google Scholar]
  4. Bénazet F., Guillaume L. Metronidazole and nitrimidazine. Lancet. 1972 Mar 11;1(7750):597–597. doi: 10.1016/s0140-6736(72)90394-7. [DOI] [PubMed] [Google Scholar]
  5. COSAR C., JULOU L. Activité de l'(hydroxy-2'éthyl)-1 méthyl-2 nitro-5 imidazole (8.823 R. P.) vis-à-vis des infections expérimentales à Trichomonas vaginalis. Ann Inst Pasteur (Paris) 1959 Feb;96(2):238–241. [PubMed] [Google Scholar]
  6. Cantone A., De Carneri I., Emanueli A., Giraldi P. N., Logemann W., Longo R., Meinardi G., Monti G., Nannini G., Tosolini G. La Nitrimidazina, un nuovo tricomonacida sistemico. G Mal Infett Parassit. 1969 Dec;21(12):954–958. [PubMed] [Google Scholar]
  7. Capet R. G. Amelioration des milieux de culture du trichomonas en vue du diagnostic rapide. Can J Public Health. 1968 May;59(5):201–203. [PubMed] [Google Scholar]
  8. Csonka G. W. Trichomonal vaginitis treated with one dose of metronidazole. Br J Vener Dis. 1971 Dec;47(6):456–458. doi: 10.1136/sti.47.6.456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. De Carneri I., Achilli G., Monti G., Trane F. Induction of in-vivo resistance of trichomonas vaginalis to metronidazole. Lancet. 1969 Dec 13;2(7633):1308–1309. doi: 10.1016/s0140-6736(69)90849-6. [DOI] [PubMed] [Google Scholar]
  10. Forsgren A. Influence of normal human serum on the determination of trichomonicidal drug concentrations. Br J Vener Dis. 1972 Jun;48(3):205–206. doi: 10.1136/sti.48.3.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Glahn B. E. Diagnostik af trichomoniasis. En sammenligning af metoder. Ugeskr Laeger. 1973 Jun 18;135(25):1293–1295. [PubMed] [Google Scholar]
  12. Howes H. L., Jr, Lynch J. E., Kivlin J. L. Tinidazole, a new antiprotozoal agent: Effect on Trichomonas and other protozoa. Antimicrob Agents Chemother (Bethesda) 1969;9:261–266. [PubMed] [Google Scholar]
  13. JENNISON R. F., STENTON P., WATT L. Laboratory studies with the systemic trichomonacide, metronidazole. J Clin Pathol. 1961 Jul;14:431–435. doi: 10.1136/jcp.14.4.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jeney E., Zsolnai T. Die antimikrobieelle Wirkung von neueren Aminoguanidonen. Zentralbl Bakteriol Orig. 1969 May;210(1):107–114. [PubMed] [Google Scholar]
  15. KANE P. O., McFADZEAN J. A., SQUIRES S., KING A. J., NICOL C. S. Absorption and excretion of metronidazole. I. Serum concentration and urinary excretion after oral administration. Br J Vener Dis. 1961 Dec;37:273–275. doi: 10.1136/sti.37.4.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Keighley E. E. Trichomoniasis in a closed community: efficacy of metronidazole. Br Med J. 1971 Jan 23;1(5742):207–209. doi: 10.1136/bmj.1.5742.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McFadzean J. A., Pugh I. M., Squires S. L., Whelan J. P. Further observations on strain sensitivity of Trichomonas vaginalis to metronidazole. Br J Vener Dis. 1969 Jun;45(2):161–162. doi: 10.1136/sti.45.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Paredes F. R., Hawkins D. F. Sensitivity of Trichomonas vaginalis to chemotherapeutic agents. J Obstet Gynaecol Br Commonw. 1973 Jan;80(1):86–88. doi: 10.1111/j.1471-0528.1973.tb02138.x. [DOI] [PubMed] [Google Scholar]
  19. Prince H. N., Grunberg E., Titsworth E., DeLorenzo W. F. Effects of l-(2-nitro-l-imidazolyl)-3-methoxy-2-propanol and 2-methyl-5-nitroimidazole-l-ethanol against anaerobic and aerobic bacteria and protozoa. Appl Microbiol. 1969 Nov;18(5):728–730. doi: 10.1128/am.18.5.728-730.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SCURI R., FAILLA L. PROPRIETA' BIOLOGICHE DELL'N-(5-NITRO-2-FURFURILIDENE)-3-AMINO-5-METILMERCAPTOMETIL-2-OSSAZOLIDINONE. Farmaco Sci. 1964 Apr;19:301–311. [PubMed] [Google Scholar]
  21. SQUIRES S., McFADZEAN J. A. Strain sensitivity of Trichomonas vaginalis to metronidazole. Br J Vener Dis. 1962 Dec;38:218–219. doi: 10.1136/sti.38.4.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Taylor J. A., Jr, Migliardi J. R., Von Wittenau M. S. Tinidazole and metronidazole pharmocokinetics in man and mouse. Antimicrob Agents Chemother (Bethesda) 1969;9:267–270. [PubMed] [Google Scholar]
  23. Tsai Y. H., Price K. E. Experimental models for the evaluation of systemic trichomonacides. Models for evaluation of trichomonacides. Chemotherapy. 1973;18(6):348–357. doi: 10.1159/000221282. [DOI] [PubMed] [Google Scholar]
  24. WATT L., JENNISON R. F. Metronidazole treatment of trichomoniasis in the female. Report of an extended trial. Br Med J. 1962 Feb 3;1(5274):276–279. doi: 10.1136/bmj.1.5274.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wood B. A., Monro A. M. Pharmacokinetics of tinidazole and metronidazole in women after single large oral doses. Br J Vener Dis. 1975 Feb;51(1):51–53. doi: 10.1136/sti.51.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]

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