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. 1984 Jun;60(3):196–198. doi: 10.1136/sti.60.3.196

Sheffield medium for cultivation of Haemophilus ducreyi.

S Hafiz, M G McEntegart, G R Kinghorn
PMCID: PMC1046300  PMID: 6610455

Abstract

Our interest in the role of Haemophilus ducreyi in genital ulceration led us to examine the various media commonly used for the cultivation of the organism. We describe an improved medium for the routine isolation of H ducreyi. In comparative studies using 50 test strains originally isolated in the United Kingdom, Canada, the United States of America, and Kenya, the new medium proved superior to three standard media in requiring a shorter incubation period to first visible growth, giving larger colonies in the same period, and making possible a starch aggregation test which we have found helpful in the presumptive identification of H ducreyi from clinical material.

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Selected References

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  1. Hafiz S., Kinghorn G. R., McEntegart M. G. Chancroid in Sheffield. A report of 22 cases diagnosed by isolating Haemophilus ducreyi in a modified medium. Br J Vener Dis. 1981 Dec;57(6):382–386. doi: 10.1136/sti.57.6.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hafiz S., Kinghorn G. R., McEntegart M. G. Starch aggregation as a presumptive test for Haemophilus ducreyi. Lancet. 1982 Oct 16;2(8303):872–872. doi: 10.1016/s0140-6736(82)90830-3. [DOI] [PubMed] [Google Scholar]
  3. Hammond G. W., Lian C. J., Wilt J. C., Ronald A. R. Comparison of specimen collection and laboratory techniques for isolation of Haemophilus ducreyi. J Clin Microbiol. 1978 Jan;7(1):39–43. doi: 10.1128/jcm.7.1.39-43.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. McEntegart M. G., Hafiz S., Kinghorn G. R. Haemophilus ducreyi infections--time for reappraisal. J Hyg (Lond) 1982 Dec;89(3):467–478. doi: 10.1017/s0022172400071035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Sottnek F. O., Biddle J. W., Kraus S. J., Weaver R. E., Stewart J. A. Isolation and identification of Haemophilus ducreyi in a clinical study. J Clin Microbiol. 1980 Aug;12(2):170–174. doi: 10.1128/jcm.12.2.170-174.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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