Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1988 Feb;86(2):598–601. doi: 10.1104/pp.86.2.598

The Urtica dioica Agglutinin Is a Complex Mixture of Isolectins 1

Els J M Van Damme 1, Willem F Broekaert 1, Willy J Peumans 1
PMCID: PMC1054529  PMID: 16665952

Abstract

Rhizomes of stinging nettle (Urtica dioica) contain a complex mixture of isolectins. Ion exchange chromatography with a high resolution fast protein liquid chromatography system revealed six isoforms which exhibit identical agglutination properties and carbohydrate-binding specificity and in addition have the same molecular structure and virtually identical biochemical properties. However, since the U. dioica agglutinin isolectins differ definitely with respect to their amino acid composition, it is likely that at least some of them are different polypeptides coded for by different genes.

Full text

PDF
598

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Felsted R. L., Egorin M. J., Leavitt R. D., Bachur N. R. Recombinations of subunits of Phaseolus vulgaris isolectins. J Biol Chem. 1977 May 10;252(9):2967–2971. [PubMed] [Google Scholar]
  2. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  3. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  4. Leavitt R. D., Felsted R. L., Bachur N. R. Biological and biochemical properties of Phaseolus vulgaris isolectins. J Biol Chem. 1977 May 10;252(9):2961–2966. [PubMed] [Google Scholar]
  5. Murphy L. A., Goldstein I. J. Physical-chemical characterization and carbohydrate-binding activity of the A and B subunits of the Bandeiraea simplificolia I isolectins. Biochemistry. 1979 Oct 30;18(22):4999–5005. doi: 10.1021/bi00589a030. [DOI] [PubMed] [Google Scholar]
  6. Peumans W. J., Stinissen H. M., Carlier A. R. Isolation and partial characterization of wheat-germ-agglutinin-like lectins from rye (Secale cereale) and barley (Hordeum vulgare) embryos. Biochem J. 1982 Apr 1;203(1):239–243. doi: 10.1042/bj2030239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Shibuya N., Goldstein I. J., Shafer J. A., Peumans W. J., Broekaert W. F. Carbohydrate binding properties of the stinging nettle (Urtica dioica) rhizome lectin. Arch Biochem Biophys. 1986 Aug 15;249(1):215–224. doi: 10.1016/0003-9861(86)90577-1. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES