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. 1988 Apr;86(4):1005–1007. doi: 10.1104/pp.86.4.1005

Alterations in Membrane Protein-Profile during Cold Treatment of Alfalfa 1

Shyam S Mohapatra 1,2, Ronald J Poole 1,2, Rajinder S Dhindsa 1,2
PMCID: PMC1054618  PMID: 16666022

Abstract

Changes in pattern of membrane proteins during cold acclimation of alfalfa have been examined. Cold acclimation for 2 to 3 days increases membrane protein content. Labeling of membrane proteins in vivo with [35S]methionine indicates increases in the rate of incorporation as acclimation progresses. Cold acclimation induces the synthesis of about 10 new polypeptides as shown by SDS-PAGE and fluorography of membrane proteins labeled in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Gusta L. V., Weiser C. J. Nucleic Acid and protein changes in relation to cold acclimation and freezing injury of korean boxwood leaves. Plant Physiol. 1972 Jan;49(1):91–96. doi: 10.1104/pp.49.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Guy C. L., Haskell D. Induction of freezing tolerance in spinach is associated with the synthesis of cold acclimation induced proteins. Plant Physiol. 1987 Jul;84(3):872–878. doi: 10.1104/pp.84.3.872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  5. Mohapatra S. S., Poole R. J., Dhindsa R. S. Changes in Protein Patterns and Translatable Messenger RNA Populations during Cold Acclimation of Alfalfa. Plant Physiol. 1987 Aug;84(4):1172–1176. doi: 10.1104/pp.84.4.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Travis R. L., Berkowitz R. L. Characterization of Soybean Plasma Membrane during Development: FREE STEROL COMPOSITION AND CONCANAVALIN A BINDING STUDIES. Plant Physiol. 1980 May;65(5):871–879. doi: 10.1104/pp.65.5.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Uemura M., Yoshida S. Involvement of Plasma Membrane Alterations in Cold Acclimation of Winter Rye Seedlings (Secale cereale L. cv Puma). Plant Physiol. 1984 Jul;75(3):818–826. doi: 10.1104/pp.75.3.818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Yoshida S. Chemical and Biophysical Changes in the Plasma Membrane during Cold Acclimation of Mulberry Bark Cells (Morus bombycis Koidz. cv Goroji). Plant Physiol. 1984 Sep;76(1):257–265. doi: 10.1104/pp.76.1.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Yoshida S., Uemura M. Protein and Lipid Compositions of Isolated Plasma Membranes from Orchard Grass (Dactylis glomerata L.) and Changes during Cold Acclimation. Plant Physiol. 1984 May;75(1):31–37. doi: 10.1104/pp.75.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. de la Roche I. A., Pomeroy M. K., Andrews C. J. Changes in fatty acid composition in wheat cultivars of contrasting hardiness. Cryobiology. 1975 Oct;12(5):506–512. doi: 10.1016/0011-2240(75)90032-2. [DOI] [PubMed] [Google Scholar]

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