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. 1988 Oct;88(2):289–294. doi: 10.1104/pp.88.2.289

Water Deficit-Induced Changes in Abscisic Acid, Growth, Polysomes, and Translatable RNA in Soybean Hypocotyls

Robert J Bensen 1,2, John S Boyer 1,2,1, John E Mullet 1,2
PMCID: PMC1055570  PMID: 16666297

Abstract

Soybean seedlings (Glycine max L.) were germinated and dark-grown in water-saturated vermiculite (water potential = −0.01 megapascal) for 48 hours, then transferred either to water-saturated vermiculite or to low water potential vermiculite (water potential = −0.30 megapascal). A decrease in growth rate was detectable within 0.8 hour post-transfer to low water potential vermiculite. A fourfold increase in the abscisic acid content of the elongating region was observed within 0.5 hour. At 24 hours post-transfer, hypocotyl elongation was severely arrested and abscisic acid reached its highest measured level: 3.7 nanograms per milligram dry weight (74-fold increase). A comparison of the polyA+ RNA populations isolated at 24 hours post-transfer from the elongating region of water-saturated and low water potential vermiculite-grown seedlings was made by two-dimensional (isoelectric focusing-sodium dodecyl sulfate) polyacrylamide gel analysis of in vitro translation products. It revealed both increases and decreases in the relative amounts of a number of translation products. Rewatering seedlings grown in low water potential vermiculite at 24 hours post-transfer led to a total recovery in growth rate within 0.5 hour, while abscisic acid in the elongating hypocotyl region required 1 to 2 hours to return to uninduced levels. Application of 1.0 millimolar (±) abscisic acid to well-watered seedlings resulted in a 48% reduction in hypocotyl growth rate during the first 2 hours after treatment. Plants treated with abscisic acid for 24 hours had a lower polysome content than control plants. However, hypocotyl growth inhibition in abscisic acid-treated seedlings preceded the decline in polysome content.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  2. Boyer J. S., Bowen B. L. Inhibition of oxygen evolution in chloroplasts isolated from leaves with low water potentials. Plant Physiol. 1970 May;45(5):612–615. doi: 10.1104/pp.45.5.612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bozarth C. S., Mullet J. E., Boyer J. S. Cell wall proteins at low water potentials. Plant Physiol. 1987 Sep;85(1):261–267. doi: 10.1104/pp.85.1.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bray E. A., Zeevaart J. A. Compartmentation and equilibration of abscisic Acid in isolated xanthium cells. Plant Physiol. 1986 Jan;80(1):105–109. doi: 10.1104/pp.80.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cavalieri A. J., Boyer J. S. Water potentials induced by growth in soybean hypocotyls. Plant Physiol. 1982 Feb;69(2):492–496. doi: 10.1104/pp.69.2.492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Glinka Z. Abscisic Acid promotes both volume flow and ion release to the xylem in sunflower roots. Plant Physiol. 1980 Mar;65(3):537–540. doi: 10.1104/pp.65.3.537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mason H. S., Mullet J. E., Boyer J. S. Polysomes, Messenger RNA, and Growth in Soybean Stems during Development and Water Deficit. Plant Physiol. 1988 Mar;86(3):725–733. doi: 10.1104/pp.86.3.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  9. Schopfer P., Plachy C. Control of Seed Germination by Abscisic Acid : III. Effect on Embryo Growth Potential (Minimum Turgor Pressure) and Growth Coefficient (Cell Wall Extensibility) in Brassica napus L. Plant Physiol. 1985 Mar;77(3):676–686. doi: 10.1104/pp.77.3.676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sondheimer E., Galson E. C., Chang Y. P., Walton D. C. Asymmetry, its importance to the action and metabolism of abscisic Acid. Science. 1971 Nov 19;174(4011):829–831. doi: 10.1126/science.174.4011.829. [DOI] [PubMed] [Google Scholar]

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