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. 1989 Feb;89(2):525–529. doi: 10.1104/pp.89.2.525

Detection of Norspermidine and Norspermine in Medicago sativa L. (Alfalfa) 1

Benjamin Rodriguez-Garay 1,2, Gregory C Phillips 1,2, Glenn D Kuehn 1,2
PMCID: PMC1055875  PMID: 16666576

Abstract

Shoot meristem tissues of alfalfa, Medicago sativa L., were found by high performance liquid chromatography analyses to contain the uncommon polyamines, norspermidine and norspermine. The chemical structures of norspermidine and norspermine, purified from alfalfa, were confirmed by comparison of mass spectra with those from authentic standards. The discovery of norspermidine and norspermine in alfalfa implicates the presence of at least two biosynthetic enzymes, a polyamine oxidase and a previously uncharacterized aminopropyltransferase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bagni N., Torrigiani P., Barbieri P. Effect of various inhibitors of polyamine synthesis on the growth of Helianthus tuberosus. Med Biol. 1981 Dec;59(5-6):403–409. [PubMed] [Google Scholar]
  2. De Rosa M., De Rosa S., Gambacorta A., Cartenì-Farina M., Zappia V. The biosynthetic pathway of new polyamines in Caldariella acidophila. Biochem J. 1978 Oct 15;176(1):1–7. doi: 10.1042/bj1760001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. De Rosa M., De Rosa S., Gambacorta A. Occurrence and characterization of new polyamines in the extreme thermophile Caldariella acidophila. Biochem Biophys Res Commun. 1976 Mar 8;69(1):253–261. doi: 10.1016/s0006-291x(76)80300-2. [DOI] [PubMed] [Google Scholar]
  4. Fujita K., Nagatsu T., Shinpo K., Maruta K., Teradaira R., Nakamura M. Improved analysis for urinary polyamines by use of high-voltage electrophoresis on paper. Clin Chem. 1980 Oct;26(11):1577–1582. [PubMed] [Google Scholar]
  5. Hamana K., Matsuzaki S. Distinct difference in the polyamine compositions of bryophyta and pteridophyta. J Biochem. 1985 Jun;97(6):1595–1601. doi: 10.1093/oxfordjournals.jbchem.a135216. [DOI] [PubMed] [Google Scholar]
  6. Hamana K., Matsuzaki S. Widespread occurrence of norspermidine and norspermine in eukaryotic algae. J Biochem. 1982 Apr;91(4):1321–1328. doi: 10.1093/oxfordjournals.jbchem.a133818. [DOI] [PubMed] [Google Scholar]
  7. Kuttan R., Radhakrishnan A. N., Spande T., Witkop B. sym-Homospermidine, a naturally occurring polyamine. Biochemistry. 1971 Feb 2;10(3):361–365. doi: 10.1021/bi00779a001. [DOI] [PubMed] [Google Scholar]
  8. Meers P., Hong K., Bentz J., Papahadjopoulos D. Spermine as a modulator of membrane fusion: interactions with acidic phospholipids. Biochemistry. 1986 Jun 3;25(11):3109–3118. doi: 10.1021/bi00359a007. [DOI] [PubMed] [Google Scholar]
  9. Slocum R. D., Kaur-Sawhney R., Galston A. W. The physiology and biochemistry of polyamines in plants. Arch Biochem Biophys. 1984 Dec;235(2):283–303. doi: 10.1016/0003-9861(84)90201-7. [DOI] [PubMed] [Google Scholar]
  10. Smith T. A. The di- and poly-amine oxidases of higher plants. Biochem Soc Trans. 1985 Apr;13(2):319–322. doi: 10.1042/bst0130319. [DOI] [PubMed] [Google Scholar]
  11. Srivenugopal K. S., Adiga P. R. Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings. Biochem J. 1980 Aug 15;190(2):461–464. doi: 10.1042/bj1900461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stillway L. W., Walle T. Identification of the unusual polyamines 3,3'-diaminodipropylamine and N,N'-bis(3-aminoproply)-1,3-propanediamine in the white shrimp Penaeus setiferus. Biochem Biophys Res Commun. 1977 Aug 8;77(3):1103–1107. doi: 10.1016/s0006-291x(77)80092-2. [DOI] [PubMed] [Google Scholar]
  13. Yamamoto S., Shinoda S., Makita M. Occurrence of norspermidine in some species of genera Vibrio and Beneckea. Biochem Biophys Res Commun. 1979 Apr 27;87(4):1102–1108. doi: 10.1016/s0006-291x(79)80021-2. [DOI] [PubMed] [Google Scholar]
  14. Yanagisawa H., Kato A., Hoshiai S., Kamiya A., Torii N. Polyamine oxidase from water hyacinth: purification and properties. Plant Physiol. 1987 Dec;85(4):906–909. doi: 10.1104/pp.85.4.906. [DOI] [PMC free article] [PubMed] [Google Scholar]

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