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. 1989 Feb;89(2):648–651. doi: 10.1104/pp.89.2.648

Raffinose Synthesis in Chlorella vulgaris Cultures after a Cold Shock 1

Graciela L Salerno 1, Horacio G Pontis 1
PMCID: PMC1055895  PMID: 16666596

Abstract

Chlorella vulgaris cultures have been submitted to a chilling shock, bringing down the growing temperature from to 24°C to 4°C. Growth was stopped immediately, and concomitantly there was an accumulation of sucrose and a decrease in the starch content. The enzymes involved in sucrose metabolism were differentially affected by the chilling shock. Sucrose phosphate synthase activity increased while sucrose synthase was not affected. Simultaneously with the chilling shock, raffinose began to accumulate. When algal cultures were returned at 24°C, raffinose disappeared. The presence of raffinose in algal cells has not been reported before.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Garber M. P., Steponkus P. L. Alterations in Chloroplast Thylakoids during Cold Acclimation. Plant Physiol. 1976 May;57(5):681–686. doi: 10.1104/pp.57.5.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. KRISMAN C. R. A method for the colorimetric estimation of glycogen with iodine. Anal Biochem. 1962 Jul;4:17–23. doi: 10.1016/0003-2697(62)90014-3. [DOI] [PubMed] [Google Scholar]
  3. Kerr P. S., Huber S. C., Israel D. W. Effect of N-source on soybean leaf sucrose phosphate synthase, starch formation, and whole plant growth. Plant Physiol. 1984 Jun;75(2):483–488. doi: 10.1104/pp.75.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. McWilliam J. R., Naylor A. W. Temperature and plant adaptation. I. Interaction of temperature and light in the synthesis of chlorophyll in corn. Plant Physiol. 1967 Dec;42(12):1711–1715. doi: 10.1104/pp.42.12.1711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Pontis H. G., Babio J. R., Salerno G. Reversible unidirectional inhibition of sucrose synthase activity by disulfides. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6667–6669. doi: 10.1073/pnas.78.11.6667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Salerno G. L., Gamundi S. S., Pontis H. G. A procedure for the assay of sucrose synthetase and sucrose phosphate synthetase in plant homogenates. Anal Biochem. 1979 Feb;93(1):196–199. [PubMed] [Google Scholar]
  7. Steponkus P. L. Cold Acclimation of Hedera helix: Evidence for a Two Phase Process. Plant Physiol. 1971 Feb;47(2):175–180. doi: 10.1104/pp.47.2.175. [DOI] [PMC free article] [PubMed] [Google Scholar]

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