Abstract
Activity ratios and carbamylation ratios of ribulose-1,5-bisphosphate carboxylase (RuBPCase) were determined for leaves of Phaseolus vulgaris and Spinacia oleracea exposed to a variety of partial pressures of CO2 and O2 and photon flux densities (PFD). It was found that activity ratios accurately predicted carbamylation ratios except in extracts from leaves held in low PFD. In particular, it was confirmed that the loss of RuBPCase activity in low partial pressure of O2 and high PFD results from reduced carbamylation. Activity ratios of RuBPCase were lower than carbamylation ratios for Phaseolus leaves sampled in low PFD, presumably because of the presence of 2-carboxyarabinitol 1-phosphate. Spinacia leaves sampled in darkness also exhibited lower activity ratios than carbamylation ratios indicating that this species may also have an RuBPCase inhibitor even though carboxyarabinitol 1-phosphate has not been detected in this species in the past.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Evans J. R., Seemann J. R. Differences between Wheat Genotypes in Specific Activity of Ribulose-1,5-bisphosphate Carboxylase and the Relationship to Photosynthesis. Plant Physiol. 1984 Apr;74(4):759–765. doi: 10.1104/pp.74.4.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall N. P., Pierce J., Tolbert N. E. Formation of a carboxyarabinitol bisphosphate complex with ribulose bisphosphate carboxylase/oxygenase and theoretical specific activity of the enzyme. Arch Biochem Biophys. 1981 Nov;212(1):115–119. doi: 10.1016/0003-9861(81)90349-0. [DOI] [PubMed] [Google Scholar]
- McCurry S. D., Pierce J., Tolbert N. E., Orme-Johnson W. H. On the mechanism of effector-mediated activation of ribulose bisphosphate carboxylase/oxygenase. J Biol Chem. 1981 Jul 10;256(13):6623–6628. [PubMed] [Google Scholar]
- Miziorko H. M., Lorimer G. H. Ribulose-1,5-bisphosphate carboxylase-oxygenase. Annu Rev Biochem. 1983;52:507–535. doi: 10.1146/annurev.bi.52.070183.002451. [DOI] [PubMed] [Google Scholar]
- Mott K. A., Jensen R. G., O'leary J. W., Berry J. A. Photosynthesis and Ribulose 1,5-Bisphosphate Concentrations in Intact Leaves of Xanthium strumarium L. Plant Physiol. 1984 Dec;76(4):968–971. doi: 10.1104/pp.76.4.968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perchorowicz J. T., Jensen R. G. Photosynthesis and Activation of Ribulose Bisphosphate Carboxylase in Wheat Seedlings : Regulation by CO(2) and O(2). Plant Physiol. 1983 Apr;71(4):955–960. doi: 10.1104/pp.71.4.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seemann J. R., Berry J. A., Freas S. M., Krump M. A. Regulation of ribulose bisphosphate carboxylase activity in vivo by a light-modulated inhibitor of catalysis. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8024–8028. doi: 10.1073/pnas.82.23.8024. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Servaites J. C. Binding of a Phosphorylated Inhibitor to Ribulose Bisphosphate Carboxylase/Oxygenase during the Night. Plant Physiol. 1985 Aug;78(4):839–843. doi: 10.1104/pp.78.4.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharkey T. D. O(2)-insensitive photosynthesis in c(3) plants : its occurrence and a possible explanation. Plant Physiol. 1985 May;78(1):71–75. doi: 10.1104/pp.78.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharkey T. D., Seemann J. R., Berry J. A. Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Response to Changing Partial Pressure of O(2) and Light in Phaseolus vulgaris. Plant Physiol. 1986 Jul;81(3):788–791. doi: 10.1104/pp.81.3.788. [DOI] [PMC free article] [PubMed] [Google Scholar]