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. 1989 Apr;89(4):1238–1246. doi: 10.1104/pp.89.4.1238

In Vivo31P NMR Spectroscopic Studies of Soybean Bradyrhizobium Symbiosis

I. Optimization of Parameters

Dominique B Rolin 1,2, Richard T Boswell 1,2, Charles Sloger 1,2, Shu-I Tu 1,2, Philip E Pfeffer 1,2
PMCID: PMC1056002  PMID: 16666690

Abstract

31P NMR spectroscopy was used to study in vivo the symbiotic state established between soybean (Glycine max [L.] Merr. cv Williams) and Bradyrhizobium japonicum (USDA 110 and 138). Different experimental conditions were used to maintain perfused, respiring detached or attached nodules in an NMR magnet. The pH of the perfusion medium affected the cytoplasmic pH and the resolution of the spectra. The internal Pi content and distribution were assessed as a function of nodule age and green-house growth conditions and the rate of glucose and 2-deoxyglucose uptake into nodules in split and intact states. The major metabolites (glucose-6-P, fructose-1,6-diP, P-choline, Pi, NTP, UDP-glc, and NAD) were readily identified from 31P NMR spectra of perchloric acid extracts of nodules with the exception of one unknown phosphorus metabolite. Nodules stressed by glucose deprivation demonstrated movement of Pi between the vacuole and cytoplasmic compartments not previously observed in 31P NMR studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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