Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1986 Sep;82(1):61–64. doi: 10.1104/pp.82.1.61

Enzyme Dynamics of the Resurrection Plant Selaginella lepidophylla (Hook. & Grev.) Spring during Rehydration 1

James B Harten 1, William G Eickmeier 1
PMCID: PMC1056066  PMID: 16665023

Abstract

The activities of 10 enzymes involved in carbohydrate metabolism were measured in both desiccated and rehydrated fronds of the desiccation-tolerant pteridophyte Selaginella lepidophylla (Hook. & Grev.) Spring. Enzyme conservation was defined as the ratio of desiccated to hydrated frond enzyme activity. The mean level of conservation was 74%, with nine of the 10 enzymes showing significant activity increases (P<0.05) during hydration. The mean of photosynthetic enzyme conservation was significantly lower (P=0.05) than the mean for glycolytic and respiratory enzymes combined. Chloramphenicol inhibited the normal activity increase in ribulose bisphosphate carboxylase and (NADPH)triose-P dehydrogenase but not pyruvate kinase upon rehydration. Cycloheximide did not affect the normal activity increase for these three enzymes. It is concluded that substantial enzyme conservation is beneficial for rapid resumption of metabolic activity in resurrection plants.

Full text

PDF
63

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergtrom G., Schaller M., Eickmeier W. G. Ultrastructural and biochemical bases of resurrection in the drought-tolerant vascular plant, Selaginella lepidophylla. J Ultrastruct Res. 1982 Mar;78(3):269–282. doi: 10.1016/s0022-5320(82)80002-6. [DOI] [PubMed] [Google Scholar]
  2. Bovarnick J. G., Chang S. W., Schiff J. A., Schwartzbach S. D. Events surrounding the early development of Euglena chloroplasts: experiments with streptomycin in non-dividing cells. J Gen Microbiol. 1974 Jul;83(0):51–62. doi: 10.1099/00221287-83-1-51. [DOI] [PubMed] [Google Scholar]
  3. Dhindsa R. S., Bewley J. D. Messenger RNA is conserved during drying of the drought-tolerant moss Tortula ruralis. Proc Natl Acad Sci U S A. 1978 Feb;75(2):842–846. doi: 10.1073/pnas.75.2.842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Domagk G. F., Alexander W. R. D-ribose-5-phosphate isomerase from skeletal muscle. Methods Enzymol. 1975;41:424–426. doi: 10.1016/s0076-6879(75)41091-6. [DOI] [PubMed] [Google Scholar]
  5. Eickmeier W. G. Protein Synthesis and Photosynthetic Recovery in the Resurrection Plant, Selaginella lepidophylla. Plant Physiol. 1982 Jan;69(1):135–138. doi: 10.1104/pp.69.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ellis R. J., Hartley M. R. Sites of synthesis of chloroplast proteins. Nature. 1971 Oct 13;233(5320):193–196. [PubMed] [Google Scholar]
  7. Gwódź E. A., Bewley J. D. Plant desiccation and protein synthesis: an in vitro system from dry and hydrated mosses using endogenous and synthetic messenger ribonucleic Acid. Plant Physiol. 1975 Feb;55(2):340–345. doi: 10.1104/pp.55.2.340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KACHMAR J. F., BOYER P. D. Kinetic analysis of enzyme reactions. II. The potassium activation and calcium inhibition of pyruvic phosphoferase. J Biol Chem. 1953 Feb;200(2):669–682. [PubMed] [Google Scholar]
  9. Leopold A. C., Musgrave M. E., Williams K. M. Solute leakage resulting from leaf desiccation. Plant Physiol. 1981 Dec;68(6):1222–1225. doi: 10.1104/pp.68.6.1222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oliver M. J., Bewley J. D. Plant Desiccation and Protein Synthesis : V. Stability of Poly (A) and Poly (A) RNA during Desiccation and Their Synthesis upon Rehydration in the Desiccation-Tolerant Moss Tortula ruralis and the Intolerant Moss Cratoneuron filicinum. Plant Physiol. 1984 Apr;74(4):917–922. doi: 10.1104/pp.74.4.917. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Spring T. G., Wold F. Enolase from Escherichia coli. Methods Enzymol. 1975;42:323–329. doi: 10.1016/0076-6879(75)42135-8. [DOI] [PubMed] [Google Scholar]
  12. Tucker E. B., Bewley J. D. Plant Desiccation and Protein Synthesis: III. Stability of Cytoplasmic RNA during Dehydration, and Its Synthesis on Rehydration of the Moss Tortula ruralis. Plant Physiol. 1976 Apr;57(4):564–567. doi: 10.1104/pp.57.4.564. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES