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. 1986 Nov;82(3):733–738. doi: 10.1104/pp.82.3.733

Changes in Protein Synthesis in Rapeseed (Brassica napus) Seedlings during a Low Temperature Treatment 1

Luis Meza-Basso 1,2, Miren Alberdi 1,3, Monique Raynal 1, Maria-Luz Ferrero-Cadinanos 1,4, Michel Delseny 1
PMCID: PMC1056199  PMID: 16665102

Abstract

Changes induced by cold treatment in young rapeseed (Brassica napus) seedlings were investigated at the molecular level. Following germination at 18°C for 48 hours, one half of the seedlings was transferred to 0°C for another 48 hour period, the other half being kept at 18°C as a control. Newly synthesized proteins were labeled for the last 6 hours of incubation with [35S]methionine. The different polypeptides were separated by two-dimensional electrophoresis in polyacrylamide gels. Newly synthesized proteins were revealed by fluorography. Protein synthesis clearly continues at 0°C and some polypeptides preferentially accumulate at this temperature. On the other hand, synthesis of several others is repressed while many are insensitive to cold treatment. Similar changes are also observed when mRNA is prepared from cold treated seedlings, translated in vitro in a reticulocyte cell free system and compared with the products of mRNA extracted from control samples. Among the genes which are repressed we identified the small subunit of ribulose 1,6-bisphosphate carboxylase. These changes are also detectable after shorter treatments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dennis E. S., Gerlach W. L., Pryor A. J., Bennetzen J. L., Inglis A., Llewellyn D., Sachs M. M., Ferl R. J., Peacock W. J. Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize. Nucleic Acids Res. 1984 May 11;12(9):3983–4000. doi: 10.1093/nar/12.9.3983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Guy C. L., Niemi K. J., Brambl R. Altered gene expression during cold acclimation of spinach. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3673–3677. doi: 10.1073/pnas.82.11.3673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Key J. L., Lin C. Y., Chen Y. M. Heat shock proteins of higher plants. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3526–3530. doi: 10.1073/pnas.78.6.3526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Meyer Y., Chartier Y. Long-lived and short-lived heat-shock proteins in tobacco mesophyll protoplasts. Plant Physiol. 1983 May;72(1):26–32. doi: 10.1104/pp.72.1.26. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  8. Roughan P. G. Phosphatidylglycerol and chilling sensitivity in plants. Plant Physiol. 1985 Mar;77(3):740–746. doi: 10.1104/pp.77.3.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sachs M. M., Freeling M., Okimoto R. The anaerobic proteins of maize. Cell. 1980 Jul;20(3):761–767. doi: 10.1016/0092-8674(80)90322-0. [DOI] [PubMed] [Google Scholar]
  10. Sarhan F., Chevrier N. Regulation of RNA Synthesis by DNA-Dependent RNA Polymerases and RNases during Cold Acclimation in Winter and Spring Wheat. Plant Physiol. 1985 Jun;78(2):250–255. doi: 10.1104/pp.78.2.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Uemura M., Yoshida S. Involvement of Plasma Membrane Alterations in Cold Acclimation of Winter Rye Seedlings (Secale cereale L. cv Puma). Plant Physiol. 1984 Jul;75(3):818–826. doi: 10.1104/pp.75.3.818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Vernet T., Fleck J., Durr A., Fritsch C., Pinck M., Hirth L. Expression of the gene coding for the small subunit of ribulosebisphosphate carboxylase during differentiation of tobacco plant protoplasts. Eur J Biochem. 1982 Sep 1;126(3):489–494. doi: 10.1111/j.1432-1033.1982.tb06806.x. [DOI] [PubMed] [Google Scholar]
  13. Vierling E., Key J. L. Ribulose 1,5-Bisphosphate Carboxylase Synthesis during Heat Shock. Plant Physiol. 1985 May;78(1):155–162. doi: 10.1104/pp.78.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]

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