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. 1987 Jul;84(3):937–943. doi: 10.1104/pp.84.3.937

Calmodulin mRNA in Barley (Hordeum vulgare L.) 1

Apparent Regulation by Cell Proliferation and Light

Raymond E Zielinski 1
PMCID: PMC1056698  PMID: 16665547

Abstract

Calmodulin is encoded by a 650-nucleotide mRNA in higher plants. This messenger was identified in barley and pea by a combination of in vitro translation and blot hybridization experiments using anti-sense RNA produced from an eel calmodulin cDNA probe. In all plant tissues tested, calmodulin mRNA represents between 0.01 and 0.1% of the total translatable mRNA population. Calmodulin mRNA levels are three- to fourfold higher in the meristematic zone of the first leaf of barley. At all other stages of leaf cell differentiation, calmodulin mRNA levels are nearly identical. During light-induced development in barley leaves, the relative proportion of translatable calmodulin mRNA declines about twofold. Cytoplasmic mRNAs that may encode calmodulin-like proteins were also detected. The levels of several of these putative Ca2+-binding protein mRNAs are modulated during the course of light-induced barley leaf cell development.

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