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. 1987 Aug;84(4):1319–1324. doi: 10.1104/pp.84.4.1319

Nitrogen Enhancement of Phosphate Transport in Roots of Zea mays L

II. Kinetic and Inhibitor Studies

Frank W Smith 1,2, William A Jackson 1,2
PMCID: PMC1056772  PMID: 16665605

Abstract

Exposure to 1 millimolar ammonium pretreatments increased Vmax for phosphorous uptake in dark-grown decapitated maize seedlings without a statistically measurable change in Km. Sulfate uptake also was stimulated. The stimulation in phosphorous uptake due to ammonium pretreatment was greater in seedlings grown without phosphorous than in those grown with 25 micromolar phosphorous. The stimulus was not expressed unless the entire root system was pretreated with ammonium, and pretreatment of a part of the root system inhibited phosphorous uptake by the remaining part unless it also had been pretreated. Pretreatment with the amino acid analogs p-fluoro-dl-phenylalanine and l-azetidine-2-carboxylic acid (AZ) restricted phosphorous uptake in seedlings that were pretreated with ammonium and in those that were not, but the effect of ammonium pretreatment was not completely eliminated by the analogs. In general, translocation of the entering phosphorous was affected similarly to uptake by experimental treatments. Enhanced translocation, however, was not sufficient to account quantitatively for the increase in uptake, and an increased uptake was still evident when translocation was completely prevented by 50 micromolar AZ pretreatment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Lin W. Potassium and Phosphate Uptake in Corn Roots: Further Evidence for an Electrogenic H/K Exchanger and an OH/Pi Antiporter. Plant Physiol. 1979 May;63(5):952–955. doi: 10.1104/pp.63.5.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Pitman M. G., Wildes R. A., Schaefer N., Wellfare D. Effect of azetidine 2-carboxylic Acid on ion uptake and ion release to the xylem of excised barley roots. Plant Physiol. 1977 Aug;60(2):240–246. doi: 10.1104/pp.60.2.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Smith F. W., Jackson W. A. Nitrogen Enhancement of Phosphate Transport in Roots of Zea mays L. : I. Effects of Ammonium and Nitrate Pretreatment. Plant Physiol. 1987 Aug;84(4):1314–1318. doi: 10.1104/pp.84.4.1314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ullrich-Eberius C. I., Novacky A., Fischer E., Lüttge U. Relationship between Energy-dependent Phosphate Uptake and the Electrical Membrane Potential in Lemna gibba G1. Plant Physiol. 1981 Apr;67(4):797–801. doi: 10.1104/pp.67.4.797. [DOI] [PMC free article] [PubMed] [Google Scholar]

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