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. 1987 Aug;84(4):1431–1436. doi: 10.1104/pp.84.4.1431

Localization in Sucrose Gradients of the Pyrophosphate-Dependent Proton Transport of Maize Root Membranes

Alain Chanson 1, Paul-Emile Pilet 1
PMCID: PMC1056791  PMID: 16665622

Abstract

A maize (Zea mays L. cv LG 11) root homogenate was prepared and centrifuged to sediment the mitochondria. The pellet (6 KP) and the supernatant (6 KS) were collected and fractionated on linear sucrose density gradients. Marker enzymes were used to study the distribution of the different cell membranes in the gradients. The distribution of the ATP- and pyrophosphate-dependent proton pumping activities was similar after 3 hours of centrifugation of the 6 KS or the 6 KP fraction. The pumps were clearly separated from the mitochondrial marker cytochrome c oxidase and the plasmalemma marker UDP-glucose-sterolglucosyl-transferase. The pyrophosphate-dependent proton pump might be associated with the tonoplast, as the ATP-dependent pump, despite the lack of a specific marker for this membrane. However, under all the conditions tested, the two pumps overlapped the Golgi markers latent UDPase and glucan synthase I and the ER marker NADH-cytochrome c reductase. It is therefore not possible to exclude the presence of proton pumping activities on the Golgi or the ER of maize root cells. The two pumps (but especially the pyrophosphate-dependent one) were more active (or more abundant) in the tip than in the basal part of maize roots, indicating that these activities might be important in growth processes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ali M. S., Akazawa T. Association of H-Translocating ATPase in the Golgi Membrane System from Suspension-Cultured Cells of Sycamore (Acer pseudoplatanus L.). Plant Physiol. 1986 May;81(1):222–227. doi: 10.1104/pp.81.1.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bennett A. B., O'neill S. D., Spanswick R. M. H-ATPase Activity from Storage Tissue of Beta vulgaris: I. Identification and Characterization of an Anion-Sensitive H-ATPase. Plant Physiol. 1984 Mar;74(3):538–544. doi: 10.1104/pp.74.3.538. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boller T., Kende H. Hydrolytic enzymes in the central vacuole of plant cells. Plant Physiol. 1979 Jun;63(6):1123–1132. doi: 10.1104/pp.63.6.1123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  5. Chanson A., Fichmann J., Spear D., Taiz L. Pyrophosphate-driven proton transport by microsomal membranes of corn coleoptiles. Plant Physiol. 1985 Sep;79(1):159–164. doi: 10.1104/pp.79.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chanson A., McNaughton E., Taiz L. Evidence for a KCl-Stimulated, Mg-ATPase on the Golgi of Corn Coleoptiles. Plant Physiol. 1984 Oct;76(2):498–507. doi: 10.1104/pp.76.2.498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chanson A., Taiz L. Evidence for an ATP-Dependent Proton Pump on the Golgi of Corn Coleoptiles. Plant Physiol. 1985 Jun;78(2):232–240. doi: 10.1104/pp.78.2.232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Churchill K. A., Sze H. Anion-sensitive, h-pumping ATPase in membrane vesicles from oat roots. Plant Physiol. 1983 Mar;71(3):610–617. doi: 10.1104/pp.71.3.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dupont F. M., Bennett A. B., Spanswick R. M. Localization of a proton-translocating ATPase on sucrose gradients. Plant Physiol. 1982 Oct;70(4):1115–1119. doi: 10.1104/pp.70.4.1115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dupont F. M., Giorgi D. L., Spanswick R. M. Characterization of a proton-translocating ATPase in microsomal vesicles from corn roots. Plant Physiol. 1982 Dec;70(6):1694–1699. doi: 10.1104/pp.70.6.1694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mandala S., Mettler I. J., Taiz L. Localization of the proton pump of corn coleoptile microsomal membranes by density gradient centrifugation. Plant Physiol. 1982 Dec;70(6):1743–1747. doi: 10.1104/pp.70.6.1743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mandala S., Taiz L. Proton transport in isolated vacuoles from corn coleoptiles. Plant Physiol. 1985 May;78(1):104–109. doi: 10.1104/pp.78.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nagahashi J., Hiraike K. Effects of centrifugal force and centrifugation time on the sedimentation of plant organelles. Plant Physiol. 1982 Feb;69(2):546–548. doi: 10.1104/pp.69.2.546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nishimura M., Beevers H. Hydrolases in vacuoles from castor bean endosperm. Plant Physiol. 1978 Jul;62(1):44–48. doi: 10.1104/pp.62.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. O'neill S. D., Bennett A. B., Spanswick R. M. Characterization of a NO(3)-Sensitive H-ATPase from Corn Roots. Plant Physiol. 1983 Jul;72(3):837–846. doi: 10.1104/pp.72.3.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rea P. A., Poole R. J. Chromatographic resolution of h-translocating pyrophosphatase from h-translocating ATPase of higher plant tonoplast. Plant Physiol. 1986 May;81(1):126–129. doi: 10.1104/pp.81.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rea P. A., Poole R. J. Proton-Translocating Inorganic Pyrophosphatase in Red Beet (Beta vulgaris L.) Tonoplast Vesicles. Plant Physiol. 1985 Jan;77(1):46–52. doi: 10.1104/pp.77.1.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Thom M., Maretzki A., Komor E. Vacuoles from Sugarcane Suspension Cultures : I. ISOLATION AND PARTIAL CHARACTERIZATION. Plant Physiol. 1982 Jun;69(6):1315–1319. doi: 10.1104/pp.69.6.1315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wang Y., Leigh R. A., Kaestner K. H., Sze H. Electrogenic h-pumping pyrophosphatase in tonoplast vesicles of oat roots. Plant Physiol. 1986 Jun;81(2):497–502. doi: 10.1104/pp.81.2.497. [DOI] [PMC free article] [PubMed] [Google Scholar]

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