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. 1962 Nov;10(6):515–523. doi: 10.1128/am.10.6.515-523.1962

Biosynthesis of Cephalosporin C

I. Factors Affecting the Fermentation

J L Ott 1, C W Godzeski 1, D Pavey 1, J D Farran 1, D R Horton 1
PMCID: PMC1057906  PMID: 16349624

Abstract

A series of complex and synthetic media have been developed that are suitable for the production of cephalosporin C and cephalosporin N by a mutant strain of Cephalosporium (C.M.I. 49,137). dl-Methionine increased the yield of both antibiotics, with more effect on cephalosporin N. l-Cystine had a greater enhancing effect on formation of cephalosporin C than on formation of cephalosporin N in synthetic media; serine increased yields of cephalosporin C under certain conditions. Disaccharides or polysaccharides appeared to be the best source for carbohydrates. No evidence was found for precursor action such as is found in penicillin fermentations. The ability of resting cells to produce antibiotic was demonstrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABRAHAM E. P., NEWTON G. G. F., CRAWFORD K., BURTON H. S., HALE C. W. Cephalosporin N: a new type of penicillin. Nature. 1953 Feb 21;171(4347):343–343. doi: 10.1038/171343a0. [DOI] [PubMed] [Google Scholar]
  2. ABRAHAM E. P., NEWTON G. G. A comparison of the action of penicillinase on benzylpenicillin and cephalosporin N and the competitive inhibition of penicillinase by cephalosporin C. Biochem J. 1956 Aug;63(4):628–634. doi: 10.1042/bj0630628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ABRAHAM E. P., NEWTON G. G. Experiments on the degradation of cephalosporin C. Biochem J. 1956 Apr;62(4):658–665. doi: 10.1042/bj0620658. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. ABRAHAM E. P., NEWTON G. G., OLSON B. H., SCHUURMANS D. M., SCHENCK J. R., HARGIE M. P., FISHER M. W., FUSARI S. A. Identity of cephalosporin N and synnematin B. Nature. 1955 Sep 17;176(4481):551–551. doi: 10.1038/176551a0. [DOI] [PubMed] [Google Scholar]
  5. ABRAHAM E. P., NEWTON G. G. The structure of cephalesporin C. Biochem J. 1961 May;79:377–393. doi: 10.1042/bj0790377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. BATCHELOR F. R., DOYLE F. P., NAYLER J. H., ROLINSON G. N. Synthesis of penicillin: 6-aminopenicillanic acid in penicillin fermentations. Nature. 1959 Jan 24;183(4656):257–258. doi: 10.1038/183257b0. [DOI] [PubMed] [Google Scholar]
  7. BHUYAN B. K., JOHNSON M. J. Chemically defined media for synnematin production. J Bacteriol. 1958 Oct;76(4):376–384. doi: 10.1128/jb.76.4.376-384.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. BHUYAN B. K., MOHBERG J., JOHNSON M. J. Sulfur metabolism of synnematin-producing molds. J Bacteriol. 1958 Oct;76(4):393–399. doi: 10.1128/jb.76.4.393-399.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. BURTON H. S., ABRAHAM E. P. Isolation of antibiotics from a species of Cephalosporium; cephalosporins P1, P2, P3, P4, and P5. Biochem J. 1951 Dec;50(2):168–174. doi: 10.1042/bj0500168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. CRAWFORD K., HEATLEY N. G., BOYD P. F., HALE C. W., KELLEY B. K., MILLER G. A., SMITH N. Antibiotic production by a species of Cephalosporium. J Gen Microbiol. 1952 Feb;6(1-2):47–59. doi: 10.1099/00221287-6-1-2-47. [DOI] [PubMed] [Google Scholar]
  11. GOTTSHALL R. Y., ROBERTS J. M., PORTWOOD L. M., JENNINGS J. C. Synnematin, and antibiotic produced by Tilachlidium. Proc Soc Exp Biol Med. 1951 Feb;76(2):307–311. doi: 10.3181/00379727-76-18473. [DOI] [PubMed] [Google Scholar]
  12. HARVEY C. L., OLSON B. H. Chemically defined media supplemented with precursors for synnematin production. Appl Microbiol. 1958 Jul;6(4):276–280. doi: 10.1128/am.6.4.276-280.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. JEFFERY J. D., ABRAHAM E. P., NEWTON G. G. Further degradation products of cephalosporin C. Isolation and synthesis of 2-(4-amino-4-carboxybutyl)thiazole-4-carboxylic acid. Biochem J. 1960 May;75:216–223. doi: 10.1042/bj0750216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KAVANAGH F., TUNIN D., WILD G. D-methionine and the biosynthesis of cephalosporin N. Arch Biochem Biophys. 1958 Oct;77(2):268–274. doi: 10.1016/0003-9861(58)90075-4. [DOI] [PubMed] [Google Scholar]
  15. LODER B., NEWTON G. G., ABRAHAM E. P. The cephalosporin C nucleus (7-aminocephalosporanic acid) and some of its derivatives. Biochem J. 1961 May;79:408–416. doi: 10.1042/bj0790408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Loo Y. H., Skell P. S., Thornberry H. H., Ehrlich J., McGuire J. M., Savage G. M., Sylvester J. C. Assay of Streptomycin by the Paper-Disc Plate Method. J Bacteriol. 1945 Dec;50(6):701–709. [PMC free article] [PubMed] [Google Scholar]
  17. Moyer A. J., Coghill R. D. Penicillin: X. The Effect of Phenylacetic Acid on Penicillin Production. J Bacteriol. 1947 Mar;53(3):329–341. [PMC free article] [PubMed] [Google Scholar]
  18. NARA T., JOHNSON M. J. Production, purification, and characterization of synnematin. J Bacteriol. 1959 Feb;77(2):217–226. doi: 10.1128/jb.77.2.217-226.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. NEWTON G. G., ABRAHAM E. P. Cephalosporin C, a new antibiotic containing sulphur and D-alpha-aminoadipic acid. Nature. 1955 Mar 26;175(4456):548–548. doi: 10.1038/175548a0. [DOI] [PubMed] [Google Scholar]
  20. NEWTON G. G., ABRAHAM E. P. Degradation, structure and some derivatives of cephalosporin N. Biochem J. 1954 Sep;58(1):103–111. doi: 10.1042/bj0580103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. NEWTON G. G., ABRAHAM E. P. Isolation of cephalosporin C, a penicillin-like antibiotic containing D-alpha-aminoadipic acid. Biochem J. 1956 Apr;62(4):651–658. doi: 10.1042/bj0620651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. OLSON B. H., JENNINGS J. C., JUNEK A. J. Separation of synnematin into components A and B by paper chromatography. Science. 1953 Jan 23;117(3030):76–78. doi: 10.1126/science.117.3030.76. [DOI] [PubMed] [Google Scholar]
  23. PISANO M. A., OLSON B. H., SAN CLEMENTE C. L. A chemically defined medium for the growth of Cephalosporium salmosynnematum. J Bacteriol. 1954 Oct;68(4):444–449. doi: 10.1128/jb.68.4.444-449.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]

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