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. 1962 Nov;10(6):583–592. doi: 10.1128/am.10.6.583-592.1962

Continuous Culture of Ruminal Microorganisms in Chemically Defined Medium1

II. Culture Medium Studies

Loyd Y Quinn 1,2, Wise Burroughs 1,2, William C Christiansen 1,2
PMCID: PMC1057920  PMID: 13972780

Abstract

Ruminal ciliates have been grown in continuous culture in chemically defined media and in the absence of viable bacteria. Oligotrichic ruminal ciliates seem to require insoluble carbohydrates for growth; the holotrichic ciliates require soluble carbohydrates, but at low concentrations. Both groups of ciliates utilize amino acids as their principal nitrogen source when these are supplied in micromolar concentrations; at millimolar concentrations, amino acids are toxic, possibly from excessive ammonia formation arising from ciliate deaminase activity. Holotrichic ruminal ciliates are destroyed by overdeposition of amylopectin when glucose is present above 0.1% concentration in the medium. Ecological requirements of ruminal ciliates are also described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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