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. 1964 Jan;12(1):18–23. doi: 10.1128/am.12.1.18-23.1964

Comparison of Direct Plating Media for the Isolation and Enumeration of Enterococci in Certain Frozen Foods1

Mary K Burkwall 1, Paul A Hartman 1
PMCID: PMC1058057  PMID: 14106933

Abstract

A total of 15 agar media were examined for their yield, selectivity, readability, and simplicity of preparation and use. A thallium medium of Barnes was selected as the better of the high yield-fair selectivity type of medium and an azide-citrate medium of Reinbold appeared to be the better of the low yield-high selectivity type of medium. Sodium carbonate (optimal concentration, 0.20%) was found to increase recovery substantially when added to certain media, especially in the presence of 0.05% Tween 80. When these two ingredients were incorporated into a medium modified after Slanetz and Bartley, the resultant medium was superior to other media for the isolation and enumeration of enterococci in certain frozen foods, such as peas and hamburger, by the direct plating method.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHESBRO W. R., EVANS J. B. Factors affecting the growth of enterococci in highly alkaline media. J Bacteriol. 1959 Dec;78:858–862. doi: 10.1128/jb.78.6.858-862.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COLOBERT L., MORELIS P. Nouveau milieu sélectif pour l'isolement des entérocoques; d'emploi pratique pour leur recherche et leur numération dans l'eau. Ann Inst Pasteur (Paris) 1958 Jan;94(1):120–122. [PubMed] [Google Scholar]
  3. Chapman G. H. The Isolation of Streptococci from Mixed Cultures. J Bacteriol. 1944 Jul;48(1):113–114. doi: 10.1128/jb.48.1.113-114.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edwards P. R. The Biochemical Characters of Human and Animal Strains of Hemolytic Streptococci. J Bacteriol. 1932 Mar;23(3):259–266. doi: 10.1128/jb.23.3.259-266.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HARTMAN P. A. Enterococcus: coliform ratios in frozen chicken pies. Appl Microbiol. 1960 Mar;8:114–116. doi: 10.1128/am.8.2.114-116.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HARTMAN P. A., HUNTSBERGER D. V. Sampling procedures for bacterial analysis of prepared frozen foods. Appl Microbiol. 1960 Nov;8:382–386. doi: 10.1128/am.8.6.382-386.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KENNER B. A., CLARK H. F., KABLER P. W. Fecal Streptococci. I. Cultivation and enumeration of Streptococci in surface waters. Appl Microbiol. 1961 Jan;9:15–20. doi: 10.1128/am.9.1.15-20.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KJELLANDER J., NYGREN B. On the occurrence of fecal streptococci in industrial food products. Acta Pathol Microbiol Scand Suppl. 1962;Suppl 154:323–326. [PubMed] [Google Scholar]
  9. LITSKY W., MALLMANN W. L., FIFIELD C. W. A new medium for the detection of enterococci in water. Am J Public Health Nations Health. 1953 Jul;43(7):873–879. doi: 10.2105/ajph.43.7.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PAPAVASSILIOU J. Species differentiation of group D streptococci. Appl Microbiol. 1962 Jan;10:65–69. doi: 10.1128/am.10.1.65-69.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Packer R. A. The Use of Sodium Azide (NaN(3)) and Crystal Violet in a Selective Medium for Streptococci and Erysipelothrix rhusiopathiae. J Bacteriol. 1943 Oct;46(4):343–349. doi: 10.1128/jb.46.4.343-349.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SLANETZ L. W., BARTLEY C. H. Numbers of enterococci in water, sewage, and feces determined by the membrane filter technique with an improved medium. J Bacteriol. 1957 Nov;74(5):591–595. doi: 10.1128/jb.74.5.591-595.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sherman J. M. THE STREPTOCOCCI. Bacteriol Rev. 1937 Dec;1(1):3–97. doi: 10.1128/br.1.1.3-97.1937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Splittstoesser D. F., Wright R., Hucker G. J. Studies on Media for Enumerating Enterococci in Frozen Vegetables. Appl Microbiol. 1961 Jul;9(4):303–308. doi: 10.1128/am.9.4.303-308.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]

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