Abstract
Concentrated chemostatic cultures of HeLa S3-1, KB, and HEp # 2 cells have been grown in a dialysis fermentor. Stationary cell concentrations of approximately 1.2 × 106 cells per ml have been produced at rates of 15 × 10-3 to 20 × 10-3 cells per hour for as long as 40 days. The dialysis fermentor appears to be useful in controlling the effects of nutrients on the growth rate of the cultures. Theoretical considerations are offered.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- COHEN E. P., EAGLE H. A simplified chemostat for the growth of mammalian cells: characteristics of cell growth in continuous culture. J Exp Med. 1961 Feb 1;113:467–474. doi: 10.1084/jem.113.2.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
- EARLE W. R. Long-term cultivation of animal tissue cells in large cultures. Fed Proc. 1958 Dec;17(4):967–974. [PubMed] [Google Scholar]
- Eagle H. THE SUSTAINED GROWTH OF HUMAN AND ANIMAL CELLS IN A PROTEIN-FREE ENVIRONMENT. Proc Natl Acad Sci U S A. 1960 Apr;46(4):427–432. doi: 10.1073/pnas.46.4.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRAFF S., McCARTY K. S. Sustained cell culture. Exp Cell Res. 1957 Oct;13(2):348–357. doi: 10.1016/0014-4827(57)90014-9. [DOI] [PubMed] [Google Scholar]
- Gallup D. M., Gerhardt P. Dialysis Fermentor Systems for Concentrated Culture of Microorganisms. Appl Microbiol. 1963 Nov;11(6):506–512. doi: 10.1128/am.11.6.506-512.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HERBERT D., ELSWORTH R., TELLING R. C. The continuous culture of bacteria; a theoretical and experimental study. J Gen Microbiol. 1956 Jul;14(3):601–622. doi: 10.1099/00221287-14-3-601. [DOI] [PubMed] [Google Scholar]
- NOVICK A., SZILARD L. Experiments with the Chemostat on spontaneous mutations of bacteria. Proc Natl Acad Sci U S A. 1950 Dec;36(12):708–719. doi: 10.1073/pnas.36.12.708. [DOI] [PMC free article] [PubMed] [Google Scholar]