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. 1965 May;13(3):327–334. doi: 10.1128/am.13.3.327-334.1965

Abundant Microorganism in Soil1

L E Casida Jr 1
PMCID: PMC1058252  PMID: 14325269

Abstract

The presence in soil of large numbers of a catalase-negative, microaerophilic, coccoid microorganism was demonstrated. Use of media of high nutrient value, without incorporation of inhibitors, and growth in the absence of antagonistic microorganisms were utilized to isolate this organism from soil dilutions greater than those providing growth by other means. The organism described does not grow on soil extract agars and is missed by conventional counting techniques for soil organisms. On the basis of morphological and growth characteristics, this organism appears to have at least some taxonomic relationships to the families Actinomycetaceae and Mycobacteriaceae. It is proposed that this organism makes up much of the coccoid microflora of soil as observed by light and ultraviolet fluorescence microscopy.

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Selected References

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  1. CASIDA L. E., Jr On the isolation and growth of individual microbial cells from soil. Can J Microbiol. 1962 Feb;8:115–119. doi: 10.1139/m62-015. [DOI] [PubMed] [Google Scholar]
  2. SKINNER F. A., JONES P. C., MOLLISON J. E. A comparison of a direct- and a plate counting technique for the quantitative estimation of soil micro-organisms. J Gen Microbiol. 1952 May;6(3-4):261–271. doi: 10.1099/00221287-6-3-4-261. [DOI] [PubMed] [Google Scholar]

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