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. 1965 May;13(3):447–456. doi: 10.1128/am.13.3.447-456.1965

Pertussis Vaccine Testing for Freedom-from-Toxicity

Margaret Pittman 1, Claire B Cox 1
PMCID: PMC1058270  PMID: 14325286

Abstract

The results of 9.5 years of official testing of vaccines containing pertussis vaccine, plain or adsorbed with alum, Al(OH)3, or AlPO4, are reported. Toxicity was evaluated by weight changes in mice at 3 and 7 days after injection and intercurrent deaths. Toxicity was encountered during the early use of AlPO4 in pertussis vaccine products, with a special product and quadruple-antigen vaccines. Throughout the study Al(OH)3 products, few in number, were the least reactive of the adsorbed products. The slopes of regression lines of graded-dose responses reflected variations in reactivity of “nontoxic” vaccines. The U.S.-prescribed test is discussed relative to the (i) reactivity in children, (ii) causes of toxicity, (iii) other assays for pertussis vaccine toxicity, and (iv) the use of a reference vaccine in the toxicity test.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSEN E. K. Some observations made during experiments on mice inoculated with H. pertussis. Acta Pathol Microbiol Scand. 1952;31(4):546–560. doi: 10.1111/j.1699-0463.1952.tb00222.x. [DOI] [PubMed] [Google Scholar]
  2. BARTA G. Soluble protective antigen from Bordetella pertussis prepared with sodium desoxycholate. J Immunol. 1963 Jan;90:72–80. [PubMed] [Google Scholar]
  3. COHEN H. H. DEVELOPMENT OF PERTUSSIS VACCINE PRODUCTION AND CONTROL IN THE NATIONAL INSTITUTE OF PUBLIC HEALTH IN THE NETHERLANDS DURING THE YEARS 1950--1962. Antonie Van Leeuwenhoek. 1963;29:183–201. doi: 10.1007/BF02046052. [DOI] [PubMed] [Google Scholar]
  4. DOLBY J. M. The separation of the histamine-sensitizing factor from the protective antigens of Bordetella pertussis. Immunology. 1958 Oct;1(4):328–337. [PMC free article] [PubMed] [Google Scholar]
  5. JOO I., PUSZTAI Z., JUHASZ V. P. Histamine-sensitizing activity of various pertussis vaccines. Z Immun exp ther. 1961 Mar;121:143–158. [PubMed] [Google Scholar]
  6. KENDRICK P. L., UPDYKE E. L., ELDERING G. Comparison of pertussis cultures by mouse protection and virulence tests . Am J Public Health Nations Health. 1949 Feb;39(2):179–184. doi: 10.2105/ajph.39.2.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KIND L. S. The altered reactivity of mice after inoculation with Bordetella pertussis vaccine. Bacteriol Rev. 1958 Sep;22(3):173–182. doi: 10.1128/br.22.3.173-182.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KUROKAWA M., ISHIDA S., KURATSUKA K. ON THE BIOLOGICAL ASSAY OF TOXICITY OF PERTUSSIS VACCINE USING MICE. III. CONSTRUCTION OF CHARTS OF CRITICAL REGIONS. Jpn J Med Sci Biol. 1963 Aug;16:151–160. doi: 10.7883/yoken1952.16.151. [DOI] [PubMed] [Google Scholar]
  9. MUNOZ J. J. SYMPOSIUM ON RELATIONSHIP OF STRUCTURE OF MICROORGANISMS TO THEIR IMMUNOLOGICAL PROPERTIES. I. IMMUNOLOGICAL AND OTHER BIOLOGICAL ACTIVITIES OF BORDETELLA PERTUSSIS ANTIGENS. Bacteriol Rev. 1963 Dec;27:325–340. doi: 10.1128/br.27.4.325-340.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NIWA M., YAMADEYA Y., KUWAJIMA Y. LEAKAGE OF CELL COMPONENTS OF BORDETELLA PERTUSSIS. J Bacteriol. 1964 Sep;88:809–810. doi: 10.1128/jb.88.3.809-810.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. OLSON B. H., ELDERING G., GRAHAM B. STABILIZATION OF PERTUSSIS VACCINE IN THE PRESENCE OF BENZETHONIUM CHLORIDE. J Bacteriol. 1964 Mar;87:543–546. doi: 10.1128/jb.87.3.543-546.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PEKAREK J., REZABEK K. An endocrinological test for innocuity of the pertussis vaccine. J Hyg Epidemiol Microbiol Immunol. 1959;3(1):79–84. [PubMed] [Google Scholar]
  13. PITTMAN M. Effect of Haemophilus pertussis on immunological and physiological reactions. Fed Proc. 1957 Sep;16(3):867–872. [PubMed] [Google Scholar]
  14. PITTMAN M. Influence of preservatives of heat, and of irradiation on mouse protective activity and detoxification of pertussis vaccine. J Immunol. 1952 Aug;69(2):201–216. [PubMed] [Google Scholar]
  15. PITTMAN M. Instability of pertussis-vaccine component in quadruple antigen vaccine. Diphtheria and tetanus toxoids and pertussis and poliomyelitis vaccines. JAMA. 1962 Jul 7;181:25–30. doi: 10.1001/jama.1962.03050270027005. [DOI] [PubMed] [Google Scholar]

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