Abstract
Theoretical considerations illustrate the feasibility and characteristics of continuous virus cultivation in cell suspensions Physical dimensions of the virus fermentor (lysostat) in relation to optimal yields can be predetermined. Poliovirus type 1 was grown on Hela S-3-1 (Saltzman) cells in a single-stage, percolator-type lysostat, with a yield of 421 TCID50 per cell. Adenovirus type 14 was grown on KB (Eagle) cells in a two-stage lysostat with a yield of 116 TCID50 per cell.
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