Abstract
Addition of lard or sodium oleate to the medium used for lipase production by Pseudomonas fragi resulted in a decreased accumulation of lipase in the culture supernatant fluid without affecting cell growth. The production and activity of lipase was inhibited by lard, sodium oleate, and the salts of other unsaturated fatty acids. Some divalent cations, Tweens, lecithin, and bovine serum prevented oleate inhibition, but did not reverse it. Similar inhibitory actions were observed with Geotrichum candidum lipase, but not with a staphylococcal lipase or pancreatic lipase. A protective effect by protein in crude enzyme preparations is indicated. The ability of oleate to lower surface tension does not appear to be related to its ability to inhibit lipase.
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- ALFORD J. A., PIERCE D. A. PRODUCTION OF LIPASE BY PSEUDOMONAS FRAGI IN A SYNTHETIC MEDIUM. J Bacteriol. 1963 Jul;86:24–29. doi: 10.1128/jb.86.1.24-29.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Alford J. A., Smith J. L. Production of microbial lipases for the study of triglyceride structure. J Am Oil Chem Soc. 1965 Dec;42(12):1038–1040. doi: 10.1007/BF02636900. [DOI] [PubMed] [Google Scholar]
- CLEMENT G., CLEMENT J., BEZARD J. [Effect of human pancreatic lipase on mixed, symmetrical, synthetic triglycerides made up of long-chain fatty acids and of butyric acid]. Arch Sci Physiol (Paris) 1962;16:213–225. [PubMed] [Google Scholar]
- COLES R. S., LICHSTEIN H. C. THE INHIBITION OF MALIC ENZYME OF LACTOBACILLUS ARABINOSUS 17-5 BY OLEIC ACID. I. OBSERVATIONS ON THE REACTION. Arch Biochem Biophys. 1963 Nov;103:186–190. doi: 10.1016/0003-9861(63)90393-x. [DOI] [PubMed] [Google Scholar]
- COLES R. S., LICHSTEIN H. C. THE INHIBITION OF THE MALIC ENZYME OF LACTOBACILLUS ARABINOSUS 17-5 BY OLEIC ACID. II. PREVENTION OF ENZYME INHIBITION. Arch Biochem Biophys. 1963 Nov;103:191–195. doi: 10.1016/0003-9861(63)90394-1. [DOI] [PubMed] [Google Scholar]
- KATES M. SIMPLIFIED PROCEDURES FOR HYDROLYSIS OR METHANOLYSIS OF LIPIDS. J Lipid Res. 1964 Jan;5:132–135. [PubMed] [Google Scholar]
- KLAPPER J. A., Jr, CANN J. R. INHIBITION OF PEPTIC DIGESTION OF SERUM ALBUMIN BY FATTY ACIDS. Arch Biochem Biophys. 1964 Dec;108:531–534. doi: 10.1016/0003-9861(64)90438-2. [DOI] [PubMed] [Google Scholar]
- O'leary W. M. THE FATTY ACIDS OF BACTERIA. Bacteriol Rev. 1962 Dec;26(4):421–447. doi: 10.1128/br.26.4.421-447.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VADEHRA D. V., HARMON L. G. ACTION OF LIPASES OF STAPHYLOCOCCUS AUREUS ON MILK FAT. Appl Microbiol. 1965 May;13:335–339. doi: 10.1128/am.13.3.335-339.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]