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. 1989 Jul;90(3):968–971. doi: 10.1104/pp.90.3.968

Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase Protein Prevents the in Vitro Decline in Activity of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase 1

Simon P Robinson 1,2, Archie R Portis Jr 1,2
PMCID: PMC1061829  PMID: 16666906

Abstract

The rate of CO2 fixation by ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) following addition of ribulose 1,5-bisphosphate (RuBP) to fully activated enzyme, declined with first-order kinetics, resulting in 50% loss of rubisco activity after 10 to 12 minutes. This in vitro decline in rubisco activity, termed fall-over, was prevented if purified rubisco activase protein and ATP were added, allowing linear rates of CO2 fixation for up to 20 minutes. Rubisco activase could also stimulate rubisco activity if added after fallover had occurred. Gel filtration of the RuBP-rubisco complex to remove unbound RuBP allowed full activation of the enzyme, but the inhibition of activated rubisco during fallover was only partially reversed by gel filtration. Addition of alkaline phosphatase completely restored rubisco activity following fallover. The results suggest that fallover is not caused by binding of RuBP to decarbamylated enzyme, but results from binding of a phosphorylated inhibitor to the active site of rubisco. The inhibitor may be a contaminant in preparations of RuBP or may be formed on the active site but is apparently removed from the enzyme in the presence of the rubisco activase protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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