Abstract
A technique for selection of Dunaliella mutants defective in their capacity to recover from osmotic shocks has been developed. The selection is based on physical separation of mutants on density gradients. This technique takes advantage of the fact that Dunaliella cells, when exposed to osmotic shocks, initially change volume and density due to water gain or loss and subsequently recover their volume and density by readjusting their intracellular glycerol. Eight mutants that do not recover their original density following hyperosmotic shocks have been isolated. The mutants grow similar to wild type cells in 1 molar NaCl, and recover like the wild type from hypotonic shocks but are defective in recovering from hypertonic shocks. A partial characterization of one of the mutants is described.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bental M., Oren-Shamir M., Avron M., Degani H. P and C-NMR Studies of the Phosphorus and Carbon Metabolites in the Halotolerant Alga, Dunaliella salina. Plant Physiol. 1988 Jun;87(2):320–324. doi: 10.1104/pp.87.2.320. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Einspahr K. J., Peeler T. C., Thompson G. A., Jr Rapid changes in polyphosphoinositide metabolism associated with the response of Dunaliella salina to hypoosmotic shock. J Biol Chem. 1988 Apr 25;263(12):5775–5779. [PubMed] [Google Scholar]
- Maeda M., Thompson G. A., Jr On the mechanism of rapid plasma membrane and chloroplast envelope expansion in Dunaliella salina exposed to hypoosmotic shock. J Cell Biol. 1986 Jan;102(1):289–297. doi: 10.1083/jcb.102.1.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oren-Shamir M., Pick U., Avron M. Involvement of the Plasma Membrane ATPase in the Osmoregulatory Mechanism of the Alga Dunaliella salina. Plant Physiol. 1989 Apr;89(4):1258–1263. doi: 10.1104/pp.89.4.1258. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pick U., Karni L., Avron M. Determination of Ion Content and Ion Fluxes in the Halotolerant Alga Dunaliella salina. Plant Physiol. 1986 May;81(1):92–96. doi: 10.1104/pp.81.1.92. [DOI] [PMC free article] [PubMed] [Google Scholar]