Abstract
Branched-chain 2-oxo acids which are formed by transamination of leucine, isoleucine, and valine are metabolized by the peroxisomes from mung bean (Vigna radiata L.) hypocotyls. Acylcoenzyme A (CoA) thio ester intermediates of the pathways were separated by reversed-phase high performance liquid chromatography. Retention time and cochromatography of individual acyl-CoA reference standards were used for identification of the acyl-CoA esters separated from the assay mixtures. Based on the results of identification and those of kinetic experiments, pathways of the peroxisomal degradation of 2-oxoisocaproate, 2-oxoisovalerate, and 2-oxo-3-methylvalerate are suggested.
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- Behrends W., Thieringer R., Engeland K., Kunau W. H., Kindl H. The glyoxysomal beta-oxidation system in cucumber seedlings: identification of enzymes required for the degradation of unsaturated fatty acids. Arch Biochem Biophys. 1988 May 15;263(1):170–177. doi: 10.1016/0003-9861(88)90625-x. [DOI] [PubMed] [Google Scholar]
- Davies D. D., Humphrey T. J. Amino Acid recycling in relation to protein turnover. Plant Physiol. 1978 Jan;61(1):54–58. doi: 10.1104/pp.61.1.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerbling H., Gerhardt B. Oxidative decarboxylation of branched-chain 2-oxo Fatty acids by higher plant peroxisomes. Plant Physiol. 1988 Sep;88(1):13–15. doi: 10.1104/pp.88.1.13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HATCH M. D., STUMPF P. K. Fat metabolism in higher plants. XVIII. Propionate metabolism by plant tissues. Arch Biochem Biophys. 1962 Feb;96:193–198. doi: 10.1016/0003-9861(62)90398-3. [DOI] [PubMed] [Google Scholar]