Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1989 Dec;91(4):1419–1424. doi: 10.1104/pp.91.4.1419

Monoclonal Antibodies against Pectin

Recognition of a Conformation Induced by Calcium

Françoise Liners 1,2, Jean-Jacques Letesson 1,2, Christian Didembourg 1,2, Pierre Van Cutsem 1,2
PMCID: PMC1062200  PMID: 16667195

Abstract

Monoclonal antibodies have been produced that recognize a conformation of homopolygalacturonic acid (pectic acid) induced by an optimum concentration of calcium and sodium of about 1 and 150 millinormal, respectively. The epitope recognized is probably part of the dimers of pectin chains associated according to the `egg box' model.

Full text

PDF
1421

Images in this article

1421Figure 2
on p.1421

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  2. McLean I. W., Nakane P. K. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem. 1974 Dec;22(12):1077–1083. doi: 10.1177/22.12.1077. [DOI] [PubMed] [Google Scholar]
  3. McNeil M., Darvill A. G., Fry S. C., Albersheim P. Structure and function of the primary cell walls of plants. Annu Rev Biochem. 1984;53:625–663. doi: 10.1146/annurev.bi.53.070184.003205. [DOI] [PubMed] [Google Scholar]
  4. Moore P. J., Darvill A. G., Albersheim P., Staehelin L. A. Immunogold localization of xyloglucan and rhamnogalacturonan I in the cell walls of suspension-cultured sycamore cells. Plant Physiol. 1986 Nov;82(3):787–794. doi: 10.1104/pp.82.3.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Morris E. R., Powell D. A., Gidley M. J., Rees D. A. Conformations and interactions of pectins. I. Polymorphism between gel and solid states of calcium polygalacturonate. J Mol Biol. 1982 Mar 15;155(4):507–516. doi: 10.1016/0022-2836(82)90484-3. [DOI] [PubMed] [Google Scholar]
  6. Powell D. A., Morris E. R., Gidley M. J., Rees D. A. Conformations and interactions of pectins. II. Influences of residue sequence on chain association in calcium pectate gels. J Mol Biol. 1982 Mar 15;155(4):517–531. doi: 10.1016/0022-2836(82)90485-5. [DOI] [PubMed] [Google Scholar]
  7. Stearns D. J., Kurosawa S., Sims P. J., Esmon N. L., Esmon C. T. The interaction of a Ca2+-dependent monoclonal antibody with the protein C activation peptide region. Evidence for obligatory Ca2+ binding to both antigen and antibody. J Biol Chem. 1988 Jan 15;263(2):826–832. [PubMed] [Google Scholar]
  8. Stähli C., Staehelin T., Miggiano V., Schmidt J., Häring P. High frequencies of antigen-specific hybridomas: dependence on immunization parameters and prediction by spleen cell analysis. J Immunol Methods. 1980;32(3):297–304. doi: 10.1016/0022-1759(80)90194-5. [DOI] [PubMed] [Google Scholar]
  9. Towbin H., Gordon J. Immunoblotting and dot immunobinding--current status and outlook. J Immunol Methods. 1984 Sep 4;72(2):313–340. doi: 10.1016/0022-1759(84)90001-2. [DOI] [PubMed] [Google Scholar]
  10. Vreeland V. Immunocytochemical localization of the extracellular polysaccharide alginic acid in the brown seaweed, Fucus distichus. J Histochem Cytochem. 1972 May;20(5):358–367. doi: 10.1177/20.5.358. [DOI] [PubMed] [Google Scholar]
  11. Vreeland V. Localization of a cell wall polysaccharide in a brown alga with labeled antibody. J Histochem Cytochem. 1970 May;18(5):371–373. doi: 10.1177/18.5.371. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES