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. 1990 Apr;92(4):1226–1232. doi: 10.1104/pp.92.4.1226

Extended Host Range of Agrobacterium tumefaciens in the Genus Pinus1

Anne-Marie Stomp 1,2,3,4, Carol Loopstra 1,2,3,4,2, W Scott Chilton 1,2,3,4, Ronald R Sederoff 1,2,3,4, Larry W Moore 1,2,3,4
PMCID: PMC1062440  PMID: 16667394

Abstract

Two-to 4-month-old seedlings of nine pine species (Pinus eldarica Medw., Pinus elliottii Engelm., Pinus jeffreyi Grev. & Balf., Pinus lambertiana Dougl., Pinus ponderosa Laws., Pinus radiata D. Don, Pinus sylvestris L., Pinus taeda L., Pinus virginiana Mill), Douglas fir (Pseudotsuaa menziesii (Mirb.) Franco) and incense cedar (Libocedrus decurrens Torr.) were inoculated with five strains of Agrobacterium tumefaciens. Transformation occurred in all conifer species tested as determined by gall formation and opine production. The frequency of gall formation varied by host species, by bacterial strain, and was related to the age of the stem when inoculated. Galls were visible 8 to 12 weeks after inoculation and were small (often less than 2.5 millimeters in diameter). Fewer than half (230 of 502) of the galls originally formed on the trees were present after 1 year, and 26 of these grew to diameters greater than 2 centimeters. The majority of these larger galls (18 of 26) were found in P. radiata. Bacterial strain-specific opines were found in 67 of the 81 gall tissues sampled.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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