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. 1990 May;93(1):148–153. doi: 10.1104/pp.93.1.148

Phosphatidylethanolamine Synthesis by Castor Bean Endosperm 1

A Base Exchange Reaction

Sungho Shin 1, Thomas S Moore Jr 1
PMCID: PMC1062481  PMID: 16667427

Abstract

A base exchange reaction for synthesis of phosphatidylethanolamine by the endoplasmic reticulum of castor bean (Ricinus comminus L. var Hale) endosperm has been examined. The calculated Michaelis-Menten constant of the enzyme for ethanolamine was 5 micromolar and the optimal pH was 7.8 in the presence of 2 millimolar CaCl2. l-Serine, N-methylethanolamine and N,N-dimethylethanolamine all reduced ethanolamine incorporation, while d-serine and myo-inositol had little effect. These inhibitions of ethanolamine incorporation were found to be noncompetitive and ethanolamine also noncompetitively inhibited l-serine incorporation by exchange. The activity of the ethanolamine base exchange enzyme was affected by several detergents, with the best activity being obtained with the zwitterionic defjtergent 3-3-cholamidopropyl) dimethylammonio-2-hydroxyl-1-propanesulfonate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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