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. 1990 Jun;93(2):471–478. doi: 10.1104/pp.93.2.471

Electrostatic Changes in Lycopersicon esculentum Root Plasma Membrane Resulting from Salt Stress

Charles G Suhayda 1,2,3,4, John L Giannini 1,2,3,4, Donald P Briskin 1,2,3,4, Michael C Shannon 1,2,3,4
PMCID: PMC1062536  PMID: 16667490

Abstract

Salinity-induced alterations in tomato (Lypersicon esculentum Mill. cv Heinz 1350) root plasma membrane properties were studied and characterized using a membrane vesicle system. Equivalent rates of MgATP-dependent H+-transport activity were measured by quinacrine fluorescence (ΔpH) in plasma membrane vesicles isolated from control or salt-stressed (75 millimolar salt) tomato roots. However, when bis-[3-phenyl-5-oxoisoxazol-4-yl] pentamethine was used to measure MgATP-dependent membrane potential (ΔΨ) formation, salt-stressed vesicles displayed a 50% greater initial quench rate and a 30% greater steady state quench than control vesicles. This differential probe response suggested a difference in surface properties between control and salt-stressed membranes. Fluorescence titration of vesicles with the surface potential probe, 8-anilino-1-napthalenesulphonic acid (ANS) provided dissociation constants (Kd) of 120 and 76 micromolar for dye binding to control and salt-stressed vesicles, respectively. Membrane surface potentials (Ψo) of−26.0 and −13.7 millivolts were calculated for control and salt-stressed membrane vesicles from the measured Kd values and the calculated intrinsic affinity constant, Ki. The concentration of cations and anions at the surface of control and salt-stressed membranes was estimated using Ψo values and the Boltzmann equation. The observed difference in membrane surface electrostatic properties was consistent with the measured differences in K+-stimulated kinetics of ATPase activity between control and salt-stressed vesicles and by the differential ability of Cl− ions to stimulate H+-transport activity. Salinity-induced changes in plasma membrane electrostatic properties may influence ion transport across the plasma membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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