Abstract
The biosynthesis of a 36 kilodalton polypeptide of Chlamydomonas reinhardtii was induced by photoautotrophic growth on low CO2. Fractionation studies using the cell-wall-deficient strain of C. reinhardtii, CC-400, showed that this polypeptide was different from the low CO2-induced periplasmic carbonic anhydrase. In addition, the 36 kilodalton polypeptide was found to be localized in intact chloroplasts isolated from low CO2-adapting cultures. This protein may, in part, account for the different inorganic carbon uptake characteristics observed in chloroplasts isolated from high and low CO2-grown C. reinhardtii cells.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Badger M. R., Kaplan A., Berry J. A. Internal Inorganic Carbon Pool of Chlamydomonas reinhardtii: EVIDENCE FOR A CARBON DIOXIDE-CONCENTRATING MECHANISM. Plant Physiol. 1980 Sep;66(3):407–413. doi: 10.1104/pp.66.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bailly J., Coleman J. R. Effect of CO(2) Concentration on Protein Biosynthesis and Carbonic Anhydrase Expression in Chlamydomonas reinhardtii. Plant Physiol. 1988 Aug;87(4):833–840. doi: 10.1104/pp.87.4.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coleman J. R., Berry J. A., Togasaki R. K., Grossman A. R. Identification of Extracellular Carbonic Anhydrase of Chlamydomonas reinhardtii. Plant Physiol. 1984 Oct;76(2):472–477. doi: 10.1104/pp.76.2.472. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldschmidt-Clermont M., Malnoë P., Rochaix J. D. Preparation of chlamydomonas chloroplasts for the in vitro import of polypeptide precursors. Plant Physiol. 1989 Jan;89(1):15–18. doi: 10.1104/pp.89.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goyal A., Tolbert N. E. Uptake of inorganic carbon by isolated chloroplasts from air-adapted dunaliella. Plant Physiol. 1989 Apr;89(4):1264–1269. doi: 10.1104/pp.89.4.1264. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Husic H. D., Kitayama M., Togasaki R. K., Moroney J. V., Morris K. L., Tolbert N. E. Identification of Intracellular Carbonic Anhydrase in Chlamydomonas reinhardtii which Is Distinct from the Periplasmic Form of the Enzyme. Plant Physiol. 1989 Mar;89(3):904–909. doi: 10.1104/pp.89.3.904. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klein U., Chen C., Gibbs M. Photosynthetic Properties of Chloroplasts from Chlamydomonas reinhardii. Plant Physiol. 1983 Jun;72(2):488–491. doi: 10.1104/pp.72.2.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Manuel L. J., Moroney J. V. Inorganic Carbon Accumulation by Chlamydomonas reinhardtii: New Proteins are made During Adaptation to Low CO(2). Plant Physiol. 1988 Oct;88(2):491–496. doi: 10.1104/pp.88.2.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moroney J. V., Husic H. D., Tolbert N. E., Kitayama M., Manuel L. J., Togasaki R. K. Isolation and Characterization of a Mutant of Chlamydomonas reinhardtii Deficient in the CO(2) Concentrating Mechanism. Plant Physiol. 1989 Mar;89(3):897–903. doi: 10.1104/pp.89.3.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moroney J. V., Kitayama M., Togasaki R. K., Tolbert N. E. Evidence for Inorganic Carbon Transport by Intact Chloroplasts of Chlamydomonas reinhardtii. Plant Physiol. 1987 Mar;83(3):460–463. doi: 10.1104/pp.83.3.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spalding M. H., Jeffrey M. Membrane-Associated Polypeptides Induced in Chlamydomonas by Limiting CO(2) Concentrations. Plant Physiol. 1989 Jan;89(1):133–137. doi: 10.1104/pp.89.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spalding M. H., Spreitzer R. J., Ogren W. L. Carbonic Anhydrase-Deficient Mutant of Chlamydomonas reinhardii Requires Elevated Carbon Dioxide Concentration for Photoautotrophic Growth. Plant Physiol. 1983 Oct;73(2):268–272. doi: 10.1104/pp.73.2.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sueoka N. MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):83–91. doi: 10.1073/pnas.46.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Toguri T., Muto S., Miyachi S. Biosynthesis and intracellular processing of carbonic anhydrase in Chlamydomonas reinhardtii. Eur J Biochem. 1986 Aug 1;158(3):443–450. doi: 10.1111/j.1432-1033.1986.tb09773.x. [DOI] [PubMed] [Google Scholar]