Abstract
13NO3− was used to investigate patterns of NO3− influx into roots of barley plants (Hordeum vulgare L. cv Klondike) previously grown with (`induced') or without (`uninduced') a source of external NO3− ([NO3−]0). In both induced and uninduced plants, 13NO3− influx was biphasic in the range from 0.005 to 50 moles per cubic meter [NO3−]0. In the low concentration range (<1 mole per cubic meter for induced plants and <0.3 mole per cubic meter for uninduced plants), influx was saturable and Vmax and Km values for influx either increased or decreased according to NO3− pretreatment. By contrast, 13NO3− influx in the high concentration range revealed a strictly linear concentration dependence. These fluxes appeared to be mediated by a constitutive, rather than an inducible, transport system.
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