Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1984 Nov;76(3):843–845. doi: 10.1104/pp.76.3.843

Dark/Light Modulation of Ribulose Bisphosphate Carboxylase Activity in Plants from Different Photosynthetic Categories 1

J Cu V Vu 1,2,3,2, Leon H Allen Jr 1,2,3, George Bowes 1,2,3
PMCID: PMC1064386  PMID: 16663937

Abstract

Ribulose bisphosphate carboxylase/oxygenase (RuBPCase) from several plants had substantially greater activity in extracts from lightexposed leaves than dark leaves, even when the extracts were incubated in vitro with saturating HCO3− and Mg2+ concentrations. This occurred in Glycine max, Lycopersicon esculentum, Nicotiana tabacum, Panicum bisulcatum, and P. hylaeicum (C3); P. maximum (C4 phosphoenolpyruvate carboxykinase); P. milioides (C3/C4); and Bromelia pinguin and Ananas comosus (Crassulacean acid metabolism). Little or no difference between light and dark leaf extracts of RuBPCase was observed in Triticum aestivum (C3); P. miliaceum (C4 NAD malic enzyme); Zea mays and Sorghum bicolor (C4 NADP malic enzyme); Moricandia arvensis (C3/C4); and Hydrilla verticillata (submersed aquatic macrophyte). It is concluded that, in many plants, especially Crassulacean acid metabolism and C3 species, a large fraction of ribulose-1,5-bisphosphate carboxylase/oxygenase in the dark is in an inactivatable state that cannot respond to CO2 and Mg2+ activation, but which can be converted to an activatable state upon exposure of the leaf to light.

Full text

PDF
845

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bahr J. T., Jensen R. G. Activation of ribulose bisphosphate carboxylase in intact chloroplasts by CO2 and light. Arch Biochem Biophys. 1978 Jan 15;185(1):39–48. doi: 10.1016/0003-9861(78)90141-8. [DOI] [PubMed] [Google Scholar]
  3. Jordan D. B., Chollet R. Inhibition of ribulose bisphosphate carboxylase by substrate ribulose 1,5-bisphosphate. J Biol Chem. 1983 Nov 25;258(22):13752–13758. [PubMed] [Google Scholar]
  4. Laing W. A., Christeller J. T. A model for the kinetics of activation and catalysis of ribulose 1,5-bisphosphate carboxylase. Biochem J. 1976 Dec 1;159(3):563–570. doi: 10.1042/bj1590563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Littlejohn R. O., Ku M. S. Characterization of Early Morning Crassulacean Acid Metabolism in Opuntia erinacea var Columbiana (Griffiths) L. Benson. Plant Physiol. 1984 Apr;74(4):1050–1054. doi: 10.1104/pp.74.4.1050. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lorimer G. H., Badger M. R., Andrews T. J. The activation of ribulose-1,5-bisphosphate carboxylase by carbon dioxide and magnesium ions. Equilibria, kinetics, a suggested mechanism, and physiological implications. Biochemistry. 1976 Feb 10;15(3):529–536. doi: 10.1021/bi00648a012. [DOI] [PubMed] [Google Scholar]
  7. Perchorowicz J. T., Jensen R. G. Photosynthesis and Activation of Ribulose Bisphosphate Carboxylase in Wheat Seedlings : Regulation by CO(2) and O(2). Plant Physiol. 1983 Apr;71(4):955–960. doi: 10.1104/pp.71.4.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Somerville C. R., Portis A. R., Ogren W. L. A Mutant of Arabidopsis thaliana Which Lacks Activation of RuBP Carboxylase In Vivo. Plant Physiol. 1982 Aug;70(2):381–387. doi: 10.1104/pp.70.2.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Vu C. V., Allen L. H., Bowes G. Effects of Light and Elevated Atmospheric CO(2) on the Ribulose Bisphosphate Carboxylase Activity and Ribulose Bisphosphate Level of Soybean Leaves. Plant Physiol. 1983 Nov;73(3):729–734. doi: 10.1104/pp.73.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES