Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1984 Dec;76(4):865–870. doi: 10.1104/pp.76.4.865

Mechanism of Amino Acid Uptake by Sugarcane Suspension Cells 1

Roger E Wyse 1,2,2, Ewald Komor 1,2
PMCID: PMC1064396  PMID: 16663961

Abstract

The amino acid carriers in sugarcane suspension cells were characterized for amino acid specificity and the stoichiometry of proton and potassium flux during amino acid transport.

Amino acid transport by sugarcane cells is dependent upon three distinct transport systems. One system is specific for neutral amino acids and transports all neutral amino acids including glutamine, asparagine, and histidine. The uptake of neutral amino acids is coupled to the uptake of one proton per amino acid; one potassium ion leaves the cells for charge compensation. Histidine is only taken up in the neutral form so that deprotonation of the charged imidazole nitrogen has to occur prior to uptake. The basic amino acids are transported by another system as uniport with charge-compensating efflux of protons and potassium. The acidic amino acids are transported by a third system. Acidic amino acids bind to the transport site only if the distal carboxyl group is in the dissociated form (i.e. if the acidic amino acid is anionic). Two protons are withdrawn from the medium and one potassium leaves the cell for charge compensation during the uptake of acid amino acids. Common to all three uptake systems is a monovalent positively charged amino acidproton carrier complex at the transport site.

Full text

PDF
865

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cockburn M., Earnshaw P., Eddy A. A. The stoicheiometry of the absorption of protons with phosphate and L-glutamate by yeasts of the genus Saccharomyces. Biochem J. 1975 Mar;146(3):705–712. doi: 10.1042/bj1460705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Collins S. H., Jarvis A. W., Lindsay R. J., Hamilton W. A. Proton movements coupled to lactate and alanine transport in Escherichia coli: isolation of mutants with altered stoichiometry in alanine transport. J Bacteriol. 1976 Jun;126(3):1232–1244. doi: 10.1128/jb.126.3.1232-1244.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eddy A. A. Mechanisms of solute transport in selected eukaryotic micro-organisms. Adv Microb Physiol. 1982;23:1-78, 269-70. doi: 10.1016/s0065-2911(08)60335-5. [DOI] [PubMed] [Google Scholar]
  4. Franz S. L., Tattar T. A. Effect of sugars and amino acids on membrane potential in two clones of sugarcane. Plant Physiol. 1981 Jan;67(1):150–155. doi: 10.1104/pp.67.1.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kinraide T. B., Etherton B. Electrical evidence for different mechanisms of uptake for basic, neutral, and acidic amino acids in oat coleoptiles. Plant Physiol. 1980 Jun;65(6):1085–1089. doi: 10.1104/pp.65.6.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Komor E., Thom M., Maretzki A. Mechanism of uptake of L-arginine by sugar-cane cells. Eur J Biochem. 1981 Jun 1;116(3):527–533. doi: 10.1111/j.1432-1033.1981.tb05368.x. [DOI] [PubMed] [Google Scholar]
  7. Maretzki A., Thom M. Arginine and lysine transport in sugarcane cell suspension cultures. Biochemistry. 1970 Jun 23;9(13):2731–2736. doi: 10.1021/bi00815a022. [DOI] [PubMed] [Google Scholar]
  8. McDaniel C. N., Holterman R. K., Bone R. F., Wozniak P. M. Amino Acid Transport in Suspension-Cultured Plant Cells : III. COMMON CARRIER SYSTEM FOR THE UPTAKE OF l-ARGININE, l-ASPARTIC ACID, l-HISTIDINE, l-LEUCINE, AND l-PHENYLALANINE. Plant Physiol. 1982 Jan;69(1):246–249. doi: 10.1104/pp.69.1.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mitchell W. J., Booth I. R., Hamilton W. A. Quantitative analysis of proton-linked transport systems. Glutamate transport in Staphylococcus aureus. Biochem J. 1979 Nov 15;184(2):441–449. doi: 10.1042/bj1840441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oxender D. L. Membrane transport. Annu Rev Biochem. 1972;41(10):777–814. doi: 10.1146/annurev.bi.41.070172.004021. [DOI] [PubMed] [Google Scholar]
  11. Robinson S. P., Beevers H. Amino Acid transport in germinating castor bean seedlings. Plant Physiol. 1981 Sep;68(3):560–566. doi: 10.1104/pp.68.3.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Seaston A., Inkson C., Eddy A. A. The absorption of protons with specific amino acids and carbohydrates by yeast. Biochem J. 1973 Aug;134(4):1031–1043. doi: 10.1042/bj1341031. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES