Abstract
During male gametophyte development the synthesis of several proteins occurs from transcripts of the haploid genome. Alcohol dehydrogenase (ADH1), a developmentally regulated protein, was chosen for study to determine the stage at which its synthesis was initiated and the pattern of its synthesis during microsporogenesis. The ability of ADH to reduce p-nitro blue tetrazolium chloride in situ was used as an indicator of enzyme activity. Maize strains heterozygous for adh1 were utilized to provide an internal control, 50% of the grains being adh1+ and 50% being adh1−. No ADH activity was detectable when tetrads were first formed after meiosis. Activity was initially detected soon after the tetrads began to break apart but before the microspores in the tetrads had completely separated. The transcription of the adh1 gene from the haploid genome must thus occur very soon after meiosis is completed. ADH activity increases at a constant rate thereafter until microspore mitosis when an increase in the rate takes place which lasts until generative cell division. Thereafter, there is a marked decrease in the rate of accumulation of ADH activity.
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- Freeling M. Intragenic recombination in maize: pollen analysis methods and the effect of parental adh1 isoalleles. Genetics. 1976 Aug;83(4):701–717. doi: 10.1093/genetics/83.4.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson O. E. Intracistron Recombination in the Wx/wx Region in Maize. Science. 1959 Sep 25;130(3378):794–795. doi: 10.1126/science.130.3378.794. [DOI] [PubMed] [Google Scholar]
- Schwartz D., Endo T. Alcohol Dehydrogenase Polymorphism in Maize-simple and Compound Loci. Genetics. 1966 Apr;53(4):709–715. doi: 10.1093/genetics/53.4.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwartz D. Genetic control of alcohol dehydrogenase--a competition model for regulation of gene action. Genetics. 1971 Mar;67(3):411–425. doi: 10.1093/genetics/67.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singh M. B., O'neill P. M., Knox R. B. Initiation of Postmeiotic beta-Galactosidase Synthesis during Microsporogenesis in Oilseed Rape. Plant Physiol. 1985 Jan;77(1):225–228. doi: 10.1104/pp.77.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tanksley S. D., Zamir D., Rick C. M. Evidence for Extensive Overlap of Sporophytic and Gametophytic Gene Expression in Lycopersicon esculentum. Science. 1981 Jul 24;213(4506):453–455. doi: 10.1126/science.213.4506.453. [DOI] [PubMed] [Google Scholar]
- Weeden N. F., Gottlieb L. D. Distinguishing allozymes and isozymes of phosphoglucoisomerases by electrophoretic comparisons of pollen and somatic tissues. Biochem Genet. 1979 Apr;17(3-4):287–296. doi: 10.1007/BF00498969. [DOI] [PubMed] [Google Scholar]