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. 1985 Mar;77(3):626–629. doi: 10.1104/pp.77.3.626

Characterization of a Purified Photosystem II-Phycobilisome Particle Preparation from Porphyridium cruentum1

Barbara M Chereskin 1, Jenny D Clement-Metral 1,2, Elisabeth Gantt 1
PMCID: PMC1064576  PMID: 16664110

Abstract

Detergent preparations isolated from thylakoids of the red alga Porphyridium cruentum, in a sucrose, phosphate, citrate, magnesium chloride medium consist of phycobilisomes and possess high rates of photosystem II activity. Characterization of these particles shows that the O2-evolving activity is stable for several hours and the pH optimum is about 6.5 to 7.2. Response of the system to light, electron donors and acceptors, and inhibitors verify that the observed activity, measured both as O2 evolution and 2,6-dichlorophenol-indophenol reduction, is due to photosystem II. Furthermore, photosystem II is functionally coupled to the phycobilisome in this preparation since green light, absorbed by phycobilisomes of P. cruentum, is effective in promoting both O2 evolution and 2,6-dichlorophenol-indophenol reduction. Photosystem II activity declines when light with wavelengths shorter than 665 nm is removed. Both 3-(3,4-dichlorophenyl)-1,1-dimethylurea and atrazine inhibit photosystem II activity in this preparation, indicating that the herbicide binding site is a component of the photosystem II-phycobilisome particle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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