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. 1985 Apr;77(4):886–890. doi: 10.1104/pp.77.4.886

Accumulation and Subcellular Localization of α-Galactosidase-Hemagglutinin in Developing Soybean Cotyledons 1

Eliot M Herman 1,3, Leland M Shannon 1
PMCID: PMC1064625  PMID: 16664156

Abstract

We have investigated the accumulation and intracellular localization of soybean (Glycine max [L.] Merr. cv Forrest) α-galactosidase-hemagglutinin during seed development. Cotyledon tissue was embedded in Lowicryl K4M and immunocytochemical localization was accomplished through treating thin sections with α-galactosidase antisera followed by indirect labeling with protein A coupled to colloidal gold. Gold particles were localized on the Golgi apparatus and protein bodies. We interpret this to indicate that α-galactosidase-hemagglutinin is transferred to and transported through the Golgi apparatus and finally deposited within the protein body by a Golgi apparatus-mediated process.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Del Campillo E., Shannon L. M. An alpha-Galactosidase with Hemagglutinin Properties from Soybean Seeds. Plant Physiol. 1982 Mar;69(3):628–631. doi: 10.1104/pp.69.3.628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dey P. M., Wallenfels K. Isolation and characterization of alpha-galactosidase from Lens esculanta. Eur J Biochem. 1974 Dec 16;50(1):106–112. doi: 10.1111/j.1432-1033.1974.tb03877.x. [DOI] [PubMed] [Google Scholar]
  3. Hankins C. N., Kindinger J. I., Shannon L. M. Legume Lectins: I. Immunological Cross-Reactions between the Enzymic Lectin from Mung Beans and other Well Characterized Legume Lectins. Plant Physiol. 1979 Jul;64(1):104–107. doi: 10.1104/pp.64.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hankins C. N., Kindinger J. I., Shannon L. M. Legume alpha-Galactosidases Which Have Hemagglutinin Properties. Plant Physiol. 1980 Apr;65(4):618–622. doi: 10.1104/pp.65.4.618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hankins C. N., Kindinger J. I., Shannon L. M. Legume: alpha-GALACTOSIDASE FORMS DEVOID OF HEMAGGLUTININ ACTIVITY. Plant Physiol. 1980 Sep;66(3):375–378. doi: 10.1104/pp.66.3.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harpaz N., Flowers H. M., Sharon N. alpha-D-galactosidase from soybeans destroying blood-group B antigens. Purification by affinity chromatography and properties. Eur J Biochem. 1977 Jul 15;77(2):419–426. doi: 10.1111/j.1432-1033.1977.tb11682.x. [DOI] [PubMed] [Google Scholar]
  7. Herman E. M., Baumgartner B., Chrispeels M. J. Uptake and apparent digestion of cytoplasmic organelles by protein bodies (protein storage vacuoles) in mung bean cotyledons. Eur J Cell Biol. 1981 Jun;24(2):226–235. [PubMed] [Google Scholar]
  8. Horisberger M., Vonlanthen M. Ultrastructural localization of soybean agglutinin on thin sections of Glycine max (soybean) var. Altona by the gold method. Histochemistry. 1980 Feb;65(2):181–186. doi: 10.1007/BF00493167. [DOI] [PubMed] [Google Scholar]
  9. Knecht D. A., Dimond R. L. Visualization of antigenic proteins on Western blots. Anal Biochem. 1984 Jan;136(1):180–184. doi: 10.1016/0003-2697(84)90321-x. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Muller L. L., Jacks T. J. Intracellular distribution of free sugars in quiescent cottonseed. Plant Physiol. 1983 Mar;71(3):703–704. doi: 10.1104/pp.71.3.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pereira M. E., Kabat E. A., Sharon N. Immunochemical studies on the specificity of soybean agglutinin. Carbohydr Res. 1974 Oct;37(1):89–102. doi: 10.1016/s0008-6215(00)87066-4. [DOI] [PubMed] [Google Scholar]
  13. Roth J., Bendayan M., Orci L. Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem. 1978 Dec;26(12):1074–1081. doi: 10.1177/26.12.366014. [DOI] [PubMed] [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Van der Wilden W., Herman E. M., Chrispeels M. J. Protein bodies of mung bean cotyledons as autophagic organelles. Proc Natl Acad Sci U S A. 1980 Jan;77(1):428–432. doi: 10.1073/pnas.77.1.428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. zur Nieden U., Manteuffel R., Weber E., Neumann D. Dictyosomes participate in the intracellular pathway of storage proteins in developing Vicia faba cotyledons. Eur J Cell Biol. 1984 May;34(1):9–17. [PubMed] [Google Scholar]

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