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. 1985 Jun;78(2):405–410. doi: 10.1104/pp.78.2.405

Transport of NAD+ in Percoll-Purified Potato Tuber Mitochondria

Inhibition of NAD+ Influx and Efflux by N-4-Azido-2-nitrophenyl-4-aminobutyryl-3′-NAD+

Michel Neuburger 1, David A Day 1,1, Roland Douce 1
PMCID: PMC1064744  PMID: 16664254

Abstract

A mechanism by which intact potato (Solanum tuberosum) mitochondria may regulate the matrix NAD content was studied in vitro. If mitochondria were incubated with NAD+ at 25°C in 0.3 molar mannitol, 10 millimolar phosphate buffer (pH 7.4), 5 millimolar MgCl2, and 5 millimolar α-ketoglutarate, the NAD pool size increased with time. In the presence of uncouplers, net uptake was not only inhibited, but NAD+ efflux was observed instead. Furthermore, the rate of NAD+ accumulation in the matrix space was strongly inhibited by the analog N-4-azido-2-nitrophenyl-4-aminobutyryl-3′-NAD+. When suspended in a medium that avoided rupture of the outer membrane, intact purified mitochondria progressively lost their NAD+ content. This led to a slow decrease of NAD+-linked substrates oxidation by isolated mitochondria The rate of NAD+ efflux from the matrix space was strongly temperature dependent and was inhibited by the analog inhibitor of NAD+ transport indicating that a carrier was required for net flux in either direction. It is proposed that uptake and efflux operate to regulate the total matrix NAD pool size.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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